摘要
目的研究山西省艾滋病病毒Ⅰ型(HIV-1)主要流行株env基因gp120区变异特性及其分子流行病学的意义。方法应用套式聚合酶链反应(Nested-PCR)对山西省56份HIV-1毒株的gp120区进行扩增,使用ABI测序仪测序,应用BLAST、GCG和MEGA等序列分析软件,对gp120区序列进行分析。结果山西省HIV-1主要流行株在系统进化树上与国际参考株B.CN.RL42-U71182汇聚在一起。山西省56份HIV-1毒株gp120区的共享序列与同源性较高的国际参考株B.CN.RL42-U71182相比,N-糖基化位点增加了5个,并在V1-V5、C2、C3、C5区段存在独特的特征性氨基酸位点。V3顶端四肽存在着9种类型:GPGQ(39.29%)、GPGR(19.64%)、GPGK(17.86%)、GQGR(10.71%)、GLGR(5.36%),GQGQ、GPGA、RLRR与RPRK分别为1.79%。结论在山西省HIV感染者中,HIV-1主要流行株仍为B’亚型,其HIV感染者中流行毒株的基因变化较大,gp120基因N-糖基化位点的增加和特征性氨基酸的存在,以及多种V3顶端四肽的存在,表明在较长的流行时间里已经有形成自己独特的序列特征模式的倾向。
Objective To analyze the variation of HIV-1 env gene gp120 region in Shanxi province of China and the significance of its molecular epidemiology. Method gp120 full-length sequences of provirus DNA extracted from 56 HIV- 1 positive blood samples were amplified by nested-PCR, and were sequenced by ABI3100 auto sequencer and the sequences were analyzed by BLAST,GCG and MEGA soft wares. Results HIV-1 strains circulationg in Shanxi province were assembled with reference strain B. CN. RL42_U71182 in the phylogenetic tree. To compare with B. CN. RL42_ U71182, 5 N-linked glycosylations were added in the consensus sequence of 56 samples from Shanxi. Specific characteristic amino acids existed in the V1-V5, C2, C3, C5 regions of the consensus sequence of HIV-1 strains in Shanxi. The HIV-1 strains in the province had 9 types of V3 loop motif tips： GPGQ （39.29%）, GPGR （19.64%）, GPGK （17.86%）, GQGR （ 10.71% ）, GLGR（5.36 % ）, GQQ, GPGA, RLRR and RPRK（ 1.79 % ）. Conclusions B＇ subtype strains were predominant in Shanxi province. HIV-1 strains in this province lean to form the unique sequence characteristic. Appropriate prevention and control measures should be taken to prevent HIV-1 prevalence according to the complex state of diversity strains circulating in blood donor in Shanxi province.
出处
《中国艾滋病性病》
CAS
2008年第6期576-579,共4页
Chinese Journal of Aids & STD
基金
国家高科技“863”项目(2006AA02Z418)
国家十五科技攻关项目(2004BA719A01)
