摘要
采用PCR-RFLP-ApaⅠ方法检测高坡猪GH基因-119^+486 bp片段的多态性,探讨了不同基因型对其部分生产性能的影响。结果表明:产生2个等位基因A(449 bp)和B(316+133 bp),其频率分别为0.54和0.46;3种基因型:AA、AB和BB,其频率分别为0.26、0.56和0.18;除6月龄腹围和背膘厚外,其余指标值均以BB基因型最高,AA基因型的6月龄腹围和瘦肉率与BB基因型相比差异显著(P<0.05),其他指标在各基因型间差异均不显著(P>0.05)。表明,A可能是小型猪的有利等位基因,B则可能是大型猪的有利等位基因。
The polymorphism of the --119bp＋486bp fragment （growth hormone gene） of Gaopo pig was detected by PCR-Apa I-RFLP to study the effect on some production performance. The results showed that there were 3 genotypes （AA, AB and BB） and 2 alleles（A：449hp： B：316＋133bp） and the frequency of AA, AB, BB, A and B was 0.26,0.56, 0.18, 0.54 and 0.46 respectively. The index values of the BB genotype was the highest in 3 genotypes except abdomen girth and back-fat thickness of the pigs with age in 6 months. There was significant difference in abdomen girtlt and Lean percentage between AA Gaopo pig with age in 6 months and BB Gaopo pig with age in 6 months and no significant difference in other index values between AA, BB and AB genotypes, which indicates that A gene and B gene may be the favorable allele in Mini-pig and largescale pig respectively.
出处
《贵州农业科学》
CAS
2008年第6期112-113,共2页
Guizhou Agricultural Sciences
基金
贵州省自然科学基金项目"贵州香猪GH基因与生长性状的关系研究"[黔科合J字(2006)2044]
关键词
高坡猪
GH基因
多态性
生产性能
Gaopo pig
GH （growth hormone） gene
polymorphism
production performance
