摘要
选用100个RAPD引物和95对SSR引物进行PCR扩增,旨在构建42份高粱和苏丹草品种资源及2份国审品种高粱-苏丹草杂交种的DNA指纹图谱。结果表明,从100个RAPD引物中筛选到9个多态性高、重复性好的引物,多态性条带比率为64.06%,利用4个核心RAPD引物可以为每份品种构建1张特定的数字指纹,并通过其中1个引物F-01构建了1张能鉴别2个杂交种的RAPD指纹图谱,不过该图谱不能区别皖草3号与其父本Sa。从95对SSR引物中筛选出多态性丰富的引物73对,多态性条带比率为86.06%,通过3对核心SSR引物就可以构建42份高粱和苏丹草的SSR数字指纹,同时利用其中1对SSR引物txp18,寻找到2个杂交种的互补带,从而构建了2个高粱-苏丹草杂交种的SSR指纹图谱,这张SSR指纹图谱不仅能鉴别皖草2号和3号,还可以把杂交种与其亲本区别开来。
100 RAPD primers and 95 pairs of SSR primers were amplified by PCR to constructed DNA fingerprints of 42 sorghum （Sorghum bicolor） and sudangrass （S. sudanense） accessions and of two sorghum-sudangrass hybrids. The polymorphism rate of 9 RAPD primers with high polymorphism and repeatability was 64.06 %. A special data fingerprint could be constructed for each accession using four RAPD core primers. One RAPD fingerprint was constructed by primer F-01 which could differentiate the two sorghum--sudangrass hybrids, but not Wancao No. 3 and Sa. The polymorphism rate of 73 SSR primer pairs screened from 95 SSR primers was 86.06 %. A special data fingerprint could be constructed for every accession using three SSR core primer pairs. Primer txp18 showed obvious complementary bands between the two hybrids. One SSR fingerprint was constructed using primer txp18, which could distinguish Wancao No. 2, from Wancao No. 3 and their parents.
出处
《草业学报》
CSCD
2008年第6期85-92,共8页
Acta Prataculturae Sinica
基金
科技部农业科技成果转化资金项目(No:2008GB2C300125)
安徽省“十一五”科技攻关计划重点项目(No:06013104B)
安徽省高校学科拔尖人才基金(No:教秘人[2005]79号)资助
关键词
高粱
苏丹草
杂交种
RAPD
SSR
指纹图谱
sorghum （Sorghum bicolor）
sudangrass （Sorghum sudanense）
hybrid
RAPD （random amplified polymorphic DNA）
SSR （simple sequence repeat）
fingerprinting
