摘要
ＡＥＶＶａｎＲｏｅｋｅｌ鸡胚适应株在鸡胚成纤维细胞（ＣＥＦ）和鸡胚神经胶质细胞（ＣＥＢ）传代，用盲传至第６代的ＣＥＦ、ＣＥＢ制成荧光标本。建立了ＡＥＶ荧光抗体检测方法，确定了待检血清稀释度１∶１０和荧光抗体ＦＩＴＣ羊抗鸡ＩｇＧ的稀释度１∶１０的工作浓度。通过对ＮＤＶ、ＭＤＶ、ＩＢＤＶ、ＡＩＶ、Ｒｅｏ等标准阳性血清的交叉试验，证实该法特异性强，且简单、方便、经济，适合于鸡群ＡＥＶ抗体的检测。
The Van Rockel embryoadapted strain of avian encephalomyelitis virus(AEV)was blindly passaged in chicken embryo brain(CEB)cell and chicken embryo fibroblast(CEF)cell for six generations,Then the CEB and CEF cell cultures infected with AEV were made into fluorescenec slides.The working dilutions of 1∶10 for serum need to be detected and 1∶10 for fluorescent antibody FiTC sheepantichicken IgG were determined. The results of the cross experiments using standard positive sera of NDV,MDV,IBDV,Reovirus,etc.confirmed that IFAT is specific,economical,and esy to perform.It can be used to detect AEV antibody of chickens.
