摘要
对上海某鸭场13日龄急性死亡角弓反张的病鸭进行了系列诊断、病鸭肝脏病理变化典型，肉眼观察呈上黄色，且肿胀、出血；细菌分离培养结果阴性。进一步取病肝制成悬液，接种鸡胚，收集尿囊液分离病毒，并用雏鸭病毒性肝炎单抗酶标抗体（HRP－DHVAb）采用Dot－ELISA鉴定呈阳性。并将阳性病毒悬液离心、透析、去脂、蔗糖密度梯度离心，电镜观察，病毒颗粒为20～40nm的二十面体，对称无膜，形态结构与雏鸭病毒性肝炎病毒同，从而确定为鸭肝炎病毒。为上海地区快速诊断该病提供了依据。
Virus was separated and identified from 13 days ducks which were acutelydead. Their clinical signs were very typical,yellow liver appeared hemorrhaging and swelling.Bacterial culture from these livers was negative. Liver suspension was inoculated into chickenembryos and the allantoic fluid from dead embryos was identified with HRP-DHV Ah byDot-ELISA. The result was positive. Then the positive virus suspension was centrifugated,dialyzed. Finally,by sucrose density gradient centrifugation and electron microscopy, 20～40nm,vigintihedral,non-enveloped DHV particles were found.
出处
《上海农业学报》
CSCD
1998年第1期85-86,共2页
Acta Agriculturae Shanghai
