摘要
本文建立一种简单而灵敏度高的选择性聚合酶链反应（ＰＣＲ）检测乙型肝炎病毒前Ｃ区终止变异，在４７例慢性乙型肝炎中检出前Ｃ区终止变异４１例（８７％），在慢性肝炎、肝硬化和重症肝炎中的检出率分别为８２％（２８／３４）、１００％（１０／１０）和１００％（３／３），在ｅ抗原阳性和ｅ抗体阳性中的检出率分别为７８％（１１／１４）和９０％（３１／３３），结果提示，我国慢性乙型肝炎存在很高的前Ｃ区终止变异株感染率，即使是ｅ抗原阳性的慢性乙型肝炎。
A convenient and sensitive method of selective amplifica tionmethod has been developed for de- tection of HBV precore stop codon mutant. The present rate of precore stop codon mutant was totally detect- ed in 41 of 47 (87% ) chronic hepatitis B by the method. The prevalence in chronic hepatitis B with HBeAg positive and anti -HBe positive was 78% and 90% eachly. The results suggest that the infectious rate of HBV precore stop codon mutant was very high in chronic hepatitis B in our country ,even with HBeAg positive.
出处
《临床肝胆病杂志》
CAS
北大核心
1998年第1期40-42,共3页
Journal of Clinical Hepatology
