摘要
目的探讨喉癌组织傅立叶变换红外光谱（FTIR）特征及其分子水平变化，为FTIR诊断喉癌提供依据。方法应用FTIR仪检测32例喉癌患者的新鲜癌组织和癌旁正常组织，结合常规病理结果指认，总结二者的光谱特征。结果喉癌组织和癌旁正常组织的红外光谱表现出较大的差异性：（1）喉正常组织中1085cm^-1处核酸分子磷酸二脂基团VsPO2^-的对称伸缩振动谱带，在喉癌组织中向高波数方向位移，并且吸收强度增强；（2）喉癌组织中，1451cm^-1和1397cm^-1、2926cm^-1和2870cm^-1处吸收峰的相对峰强降低；（3）喉正常组织中的蛋白质酰胺谱带（1648cm^-1和1538cm^-1）、蛋白质分子中甲基的对称和反对称弯曲振动谱带（1397cm^-1和1451cm^-1）、蛋白质分子COH伸缩振动谱带（1164cm^-1）和氢键化的NH基团谱带（3286cm^-1），在喉癌组织中均向高波数方向位移。与常规病理结果比较，仅1例癌旁正常组织被VTIR指认为癌变，FTIR检测准确率达98．4％。结论VHR可以反映喉癌组织和正常组织的蛋白质、核酸和脂类等生物大分子在含量、结构和构象上的差异，揭示喉癌组织的特征，有望成为喉癌术前早期诊断、术中辅助快速诊断的一种新方法。
Objective To investigate the characteristics of freshly resected laryngeal carcinoma by Fourier transform infrared spectroscopy （FTIR）. Methods FTIR was applied to the study of thecancerous tissues and adjacent normal tissues in 32 patients. Results Compared with pathological diagnosis results, one benign specimen was classified as a malignant, the accuracy was 98.4%. Significant differences were seen in the FTIR spectra between the normal and malignant laryngeal tissues. The peak at 1085 cm^-1 shift to 1114 cm ^-1 showed that the relative contents of DNA in laryngeal carcinoma ceils was increased. The peak at 1397cm^-1 was stronger than 1451 cm^-1 in normal tissues, while it was not obvious in cancer tissues. I2926/ I2870 in carcinoma cells was lower than that in normal tissues. The wave numbers of the bands of amide Ⅰ and amide Ⅱ , symmetric and asymmetric stretching bands of CH3 , stretching vibration bands of C-OH and NH band were shifted to higher number in cancer tissues. Conclusion The study shows that the malignant and normal laryngeal tissues have different FTIR spectra, which are mainly due to changes in protein, nucleic acid and phospholipids. FTIR may become a new method for the diagnosis of laryngeal carcinoma in clinical practice.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2008年第12期901-904,共4页
Chinese Journal of Oncology
基金
浙江省卫生厅重点科技资助项目（20058135）
关键词
喉肿瘤
傅立叶变换红外光谱
诊断
Laryngeal neoplasms
Fourier transform infrared spectroscopy
Diagnosis
