摘要
目的比较三种方法对临床分离大肠埃希菌、肺炎克雷伯菌中产超广谱β-内酰胺酶(ESBLs)的检出率。方法采用VIKET2全自动微生物鉴定仪(仪器法)、纸片扩散法确证法(确证法)、双纸片扩散协同法(协同法)进行试验。结果仪器法、确证法、协同法检测ESBLs菌株阳性率分别为45.2%、51.0%和42.3%,结果差异无显著性(P>0.05)。结论仪器法、确证法和协同法对检测ESBLs菌株阳性率差异无统计学意义,各实验室可根据各自的条件而选择不同的方法。
Objective To compare three methods for detection of extended-spectrum-β-lactamases-producing （ESBLs） Escherichia Coli and Klebsiella pneumoniae. Methods ESBLs-bactreia were detected by VIKET2 AES, disc confirmatory test and double-disc synergy test. Results The ESBLs positive rate of Vitek2 AES,disc confirmatory test and double-disc synergy test were 45.2%,51.0%,and 42.3% respectively, and there were no significantly different among the three methods （P〉0.05）. Conclusions Vitek2 AES, disc confirmatory test and double-disc synergy test have no obviously change to detect ESBLs, each laboratories could choose different methods accord to its conditions.
出处
《中国热带医学》
CAS
2009年第1期152-152,128,共2页
China Tropical Medicine
关键词
产超广谱Β-内酰胺酶
大肠埃希菌
肺炎克雷伯菌
Extended-spectrum-β-lactamases-production
Escherichia Coli
Klebsiella pneumoniae
