摘要
目的研究慢性乙醇中毒对内毒素(LPS)致急性肺损伤模型的易感性。方法将35只Wistar大鼠随机分为对照组、LPS组及乙醇中毒+LPS组,分别观察2h和6h2个时间点的动脉血气、肺组织湿/干比值,ELISA法测大鼠肺组织中白介素-6(IL-6)、血管内皮生长因子(VEGF)和单核细胞趋化因子-1(MCF-1)含量变化。结果LPS组和乙醇中毒+LPS组中各时间点氧分压、氧合指数、VEGF与对照组比较明显降低(P<0.01),且乙醇中毒+LPS组与LPS组比较显著降低(P<0.05);LPS组和乙醇中毒+LPS组各时间点肺湿、干重及肺湿/干比值、IL-6和MCF-1均较对照组显著增加(P<0.01);在2h时乙醇中毒+LPS组与LPS组比较差异有统计学意义(P<0.05)。结论在慢性乙醇中毒基础上的大鼠急性肺损伤程度较无慢性乙醇中毒基础的严重而且易感。
Objective To explore the effect of chronic alcoholism on susceptibility to acute lung injury induced by endotoxin （LPS） in rats. Methods 35 Wistar rats were randomly divided into three groups, they were control group, LPS group and alcoholism ＋ LPS group, then observed the changes of arterial blood gases, wet/dry ratio of lung, and contents of IL-6, VEGF and MCF-1 in lung tissues determined by ELISA at 2 h and 6 h after experiment respectively. Results The results showed that there were obvious decrease in PaO2, PaO2/FiaO2 and VEGF, and obvious increase in wet weight, dry weight, wet/dry ratio of lung, and contents of IL-6, MCF-1 in LPS group and alcoholism ＋ LPS group both at 2 h and 6 h after experiment compared with control group （P 〈 0. 01 and 〈 0.05 respectively） ; at 2 h after experiment, there was some obvious difference between alcoholism ＋ LPS group and LPS group （P 〈 0.05 ）. Conclusions The results suggested that the acute lung injury induced by LPS in chronic alcoholism rats was more severe and susceptible.
出处
《中国工业医学杂志》
CAS
北大核心
2008年第6期361-363,共3页
Chinese Journal of Industrial Medicine
关键词
急性肺损伤
乙醇中毒
易感性
白介素-6
血管内皮生长因子
单核细胞趋化因子-1
Acute lung injury
Alcoholism
Susceptibility
Interleukin-6 （IL-6）
Vascular endothelial growth factor（VEGF）
Monocyte chemotactic factor-1 （MCF-1）
