摘要
用根癌农杆菌介导法将克隆于酵母的HAL1基因转化龙牧803苜蓿胚性愈伤组织,经2 mg/L的Basta抗性筛选及PCR检测,该基因已整合到受体基因组中,共获得了11株转基因植株。培养基耐盐性实验结果:在添加NaCl的MSO培养基上,非转基因植株在NaCl浓度高于0.6%时不能生根,逐渐死亡;转基因植株在NaCl浓度0.6%～1.0%范围内仍能生根并正常生长。由此初步证明HAL1基因已在龙牧803苜蓿中表达,并且提高了耐盐性。
The HAL1 gene cloned from yeast was introduced into alfalfa cuhivar‘Longmu 803＇ embryo eellus. After screened by 2 mg/L of Basta and PCR, the HAL1 gene was proved inserted into the host genome and 11 transgenic plantlets were obtained. A salt-tolerance evaluation was carried out and the result showed that the untransgenic plants could not root on the medium MSo with a concentration of NaCl higher than 0.6% and died gradually. Contrasted with the untransgenic plants, the transgenic plants could root in the medium with a concentration around 0.6% to 1.2% of NaCl and grew normally. This indicated that the HAL1 expressed in alfalfa and improved the salt-tolerance of alfalfa.
出处
《吉林农业科学》
CSCD
2008年第6期21-24,共4页
Journal of Jilin Agricultural Sciences
