摘要
以6份玉米品种的全基因组DNA为供体,以4份普通小麦品种为受体,借助于低能离子束介导技术完成异源遗传物质的转移,对其介导试验的当代群体的特异性进行了观察鉴定。结果表明,经过离子束注入处理后试验材料的成苗率明显地降低,这是低能离子所导致的生物学效应之一。在所有的试验材料的生育前期和中期,在每一个处理的群体内主要农艺性状上没有表现出明显的差异,而在生育后期,被处理材料的大部分农艺性状与对照没有显著差异,仅仅在4个性状,即植株的生长势、穗型、主茎分蘖状态和单穗变异上,被处理材料表现出一定的变异特征。尽管离子束注入处理和直接浸泡外源DNA的处理也会导致其当代群体内出现突变株,但其群体内的突变株数量比较少,突变率比较低。经过离子束介导处理所导致的生物学效应最明显,其群体内的突变株数量比较多,突变率比较高。
In the experiment, 6 corn varieties were taken as the donors and 4 wheat varieties as the acceptor,and the transformation of hereditary materials were completed by ion beam mediated technique. The cbaracteritics of the materials in M1 were identified. The results showed that the seedling frequency of wheat after ion beam implantation was lowered,which was one of the bio- logical effects of low energy ion beam. In early and mid growing stages, the main agronomic cha racters of the materials did not display obvious differences. In late stages, most of the agronomic characters had no differences from CK except the growth vigour,ear shape,tillering state and single ear variation. In CK2-1--CK2-4 and CK3-1--CK3-4, there was also mutation, but the mutation number was few and the mutation frequency was low. The biological effects of ion beam treatment was apparent,with more mutation plants and higher variation frequence.
出处
《河南农业科学》
CSCD
北大核心
2008年第12期23-26,共4页
Journal of Henan Agricultural Sciences
基金
国家"十五"科技攻关项目(2001BA302B)
