摘要
目的探索一种建立淤热证动物模型的方法。方法间隔24 h,从大鼠尾静脉注射大肠杆菌内毒素(LPS),观察给予内毒素后不同时间大鼠体温、凝血时间、血液黏度及动静脉短路血栓等指标的改变。结果与正常对照组比较,50μg/kg内毒素可引起大鼠体温升高、血液粘度的增加、凝血系统激活及血栓形成,且各指标变化的时相呈现一致性,最佳时间点是第2次给内毒素后2 h。结论100μg/kg内毒素间隔24 h注射,能形成实验性大鼠淤热证。
Objective To explore a method of establishing an experimental animal model mixed stasis - heat syndrome in rat in vivo. Methods The body temperature and parameters of hemorrheology were monitored at different time after the lipopolysaccharide（LPS） was treated by infusion vein through vena eaudalis twice every 24 hours in rats. Results The body temperature, viscosity of whole blood, viscosity of plasma, the activity of coagulation - fibrinolysis system were significantly increased compared with control group, and the optimal time is the 2nd hour after the last treating with LPS. Conclusion Rats treated with LPS twice every 24h with total doses of 100 μg/kg result in symptom of ＂stasis＂ and ＂heat＂ of mixed statsis -heat syndrome.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第12期2896-2897,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家"973"计划中医理论专项项目(No.2006CB504807)
江苏省科技厅自然科学基金(No.BK2007242)
关键词
淤热证
血液流变学
血小板聚集
血栓
Mixed stasis - heat syndrome
Hemorrheology
Platelet aggregation
Thrombus
