摘要
采用固相萃取法提取水样,用高效液相色谱(HPLC)测定废水中土霉素(OTC)。固相萃取小柱为Octadecyl18/18%(500mg/6mL),洗脱液为甲醇,色谱柱为EclipiceplusC18柱(3.5μm,4.6mm×100mm),流动相为乙腈∶0.01mol/L柠檬酸溶液=25∶75(v/v,pH=2.5),流速0.4mL/min,检测波长为355nm。标准曲线线性良好,其回归方程及相关系数r分别为y=64.11777+27.3984799x,r=0.99994,精密度(RSD)为0.9%～2.8%,回收率在87%～115%之间。该方法方法简便,结果可靠,灵敏度高,适用于废水中土霉素残留量检测。
This report contributes to preliminary SPE-high performance liquid chromatographic （HPLC）method for confirming oxytetracycline（OTC） in pharmaceutical wasterwater. The SPE column was waters Octadecyl C18（500 mg/6 mL）. The chromatographic column was Eclipice plus C18 （3.5 μ m, 4.6 mm×100 mm）, mobile phase was HCN ： 10 mmol/L citric acid in water =25 ： 75 （v/v, pH=2.5） , flowing rate was 0.4 mL/min, UV detecting wavelength was 355 nm. Regression equation and r was：y= 64. 117 77＋27. 398 479 9 x,r=0. 999 94, RSD was0.9%-2.8%, the recoveries of antibiotics were 87%-115%. The method is simple, sensitive and accurate to determinate the residue of tetracyclines in wasterwater.
出处
《河北化工》
2008年第12期64-65,共2页
Hebei Chemical Industry
基金
河北省科学技术研究与发展计划项目(No.07276708D)
