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ECRG2基因通过干扰uPAR与β1整合素的相互作用抑制肿瘤细胞侵袭迁移 被引量：2

ECRG2 gene regulates tumor cell migration/invasion through disrupting uPAR/β1 integrin interactions
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摘要 目的探讨ECRG2影响人成纤维肉瘤HT1080细胞侵袭迁移的分子机制。方法利用ECRG2可诱导表达系统观察该基因对uPAR与β1整合素相互作用及下游Src/MAP信号通路的影响。结果ECRG2基因通过干扰uPAR与β1整合素的相互作用、阻断uPAR/β1/Src/MAP信号通路,从而抑制HT1080细胞的侵袭迁移;ECRG2基因缺失导致uPAR/β1整合素/Src/MAP信号通路活性增强,促进HT1080细胞的侵袭迁移。结论ECRG2基因通过干扰uPAR/β1整合素/Src/MAP信号通路参与肿瘤细胞侵袭迁移的调控。 Objective To explore the molecular mechanisms of ECRG2 regulating HT1080 cell migration/invasion. Methods ECRG2 gene was overexpressed or depleted by using Tet-On inducible system. The interaction between uPAR and β1 integrin and Src/ MAP kinase activity were examined by using immunoprecipitation-Westem blotting. Results Overexpression of ECRG2 gene dismpted the interaction between uPAR and β1 integrin, blocked Src/MAP kinase pathway, thus inhibited HT1080 cell migration/invasion. Depletion of ECRG2 gene resulted in enhanced uPAR/β1 integrin/Src/MAP kinase signaling, and promoted HT1080 cell migration/invasion. Conclusion ECRG2 regulates tumor cell migration/invasion through uPAR/β1 integrin/Src/MAP kinase pathway, and may represent a novel therapeutic target for cancer.
作者 成晓龙 宋肖静 崔永萍
机构地区 山西医科大学人体解剖教研室 山西医科大学细胞生物学与遗传学教研室
出处 《山西医科大学学报》 CAS 2008年第12期1061-1063,共3页 Journal of Shanxi Medical University
基金 国家自然科学基金资助项目(30500588) 山西省自然科学基金资助项目(20051089)
关键词 ECRG2 侵袭迁移 UPAR Β1整合素 ECRG2 migration/invasion uPAR β1 integrin
分类号 R730.2 [医药卫生—肿瘤]
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参考文献8

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同被引文献19

  • 1Zhang YG,Zhang HG,Zhang GY. Panx noto ginseng saponins attenuate atherosclerosis in rats by regulating the blood lipid profile and an anti-inflammatory action[J].Clinical and Experimental Pharmacology and Physiology,2008.1238-1244.
  • 2Baldwin G,Novitsava V,Sadej R. Tetraspanin CD151 regulates glycosylation of α3β1 integrin[J].Biological Chemistry,2008.35445-35454.
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  • 4Gui Y,Bi M,Su T.Molecular cloning and characterization of a novel esophageal cancer related gene[J].Int J Oncol,2010,37(6):1521-1528.
  • 5Tang CH,Wei Y.The urokinase receptor and integrins in cancer progression[J].Cell Mol Life Sci,2008,65(12):1916-1932.
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  • 7Franco P,Vocca I,Carriero M,et al.Activation of urokinase receptor by a novel interaction between the connecting peptide region of urokinase andαvβ5[J].J Cell Sci,2006,119(16):3424-3434.
  • 8Czekay RP,Aertgeerts K,Curriden SA,et al.Plasminogen activator inhibitor-1 detaches cells from extracellular matrices by inactivating integrins[J].J Cell Biol,2003,160(5):781-791.
  • 9Sitrin RG,Johnson RG,Pan PM,et al.Lipid raft compartmentalization of urokinase receptor signaling in human neutrophils[J].Am J Respir Cell Mol Biol,2004,30:233-241.
  • 10Ahmed N,Oliva K,Wang Y,et al.Downregulation of urokinase plasminogen activator receptor expression inhibits ERK signaling with concomitant suppression of invasiveness due to loss of u PAR-β1 integrin complex in colon cancer cells[J].Br J Cancer,2003,89:374-384.

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山西医科大学学报
2008年 第12期

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