摘要
为明确虫酰肼衍生物0593对家蚕Bombyx mori的毒性,本研究采用食下毒叶法测定了虫酰肼及其衍生物0593对家蚕的毒性,观察了亚致死浓度下对家蚕生长发育的影响,并测定了虫酰肼及其衍生物0593对家蚕幼虫体内保护酶的影响,对虫酰肼0593的作用机理进行了初步探索。结果表明:虫酰肼及其衍生物0593对2龄家蚕96h的LC50值分别为1.2863和0.3364mg·L-1,属高毒级药剂;虫酰肼及其衍生物0593在亚致死剂量下对家蚕的生长发育有明显的不利性,可使幼虫历期缩短0.5～2d;处理组眠期体重、全茧量、蛹重和化蛹率与对照相比均显著降低;对4龄幼虫体内多酚氧化酶和几丁质酶也有较明显影响,虫酰肼及其衍生物0593处理家蚕,处理后6h对体内多酚氧化酶有明显激活作用,12h后表现出显著的抑制作用;虫酰肼及其衍生物0593对家蚕体内几丁质酶均有激活作用。0593对保护酶的影响较虫酰肼明显。结果提示虫酰肼及其衍生物0593对家蚕毒性高,对其生长发育和保护酶类均有不利性,不适合在桑园及其周边农田使用。
In order to definite the toxicity of tebufenozide derivative 0593 to silkworm Bombyx mori, we detected the toxicity of tebufenozide and its derivative 0593 to B. rnori with food intake method, observed the growth and development of B. moti at their respective sublethal concentration, and determined their effects on endogenous protective enzymes in B. mori larvae. Based on the experimental results, we primarily discussed the action mechanism of ebufenozide derivative 0593. The results showed that the LC50 of tebufenozide and its derivative 0593 to 2nd instar larvae of B. moti were 1. 2863 mg·L^-1 and 0. 3364mg·L^-1 after 96 h exposure, respectively, and both were of high toxicity. At the sublethal dose, the growth and development of B. moti had been obviously affected, which could shorten larval period by 0.5 - 2 d. Compared with control, larval weight during molting stage, whole cocoon weight, pupal weight and pupation rate decreased of the treatments dramatically. The PPO activity in 4th instar larvae of B. mori was activated at 6 h after treatment, and then inhibited at 12 h after treatment. Both tebufenozide and its derivative 0593 significantly activated the chitinase activity in 4th instar larvae of B. mori. 0593 showed more significant effects on endogenous protective enzymes than tebufenozide. These results indicate that tebufenozide and its derivative 0593 belong to high toxicity grade to B. mori, and have adverse effects on its development and endogenous protective enzymes. So they are not suitable to be used in and around mulberry fields.
出处
《昆虫学报》
CAS
CSCD
北大核心
2008年第11期1157-1163,共7页
Acta Entomologica Sinica
基金
国家“十一五”支撑计划课题(2006BAD08A03)
山东省自然科学基金项目(J2007D43)
关键词
家蚕
虫酰肼
虫酰肼衍生物
毒性
保护酶
Bombyx rnori
tebufenozide
tebufenozide derivative
toxicity
protective enzymes
