摘要
【目的】研究HMC毒素培养滤液对玉米叶片诱导抗病性作用及抗病性相关酶的影响。【方法】以两对同核异质玉米自交系(B37和C103)为试材,采用离体叶片法检测不同稀释倍数的HMC毒素培养滤液对专化寄主玉米叶片的致病性,从中筛选适宜诱导的有效浓度,在诱导中和接种后两个阶段分别测定防御酶活性及与抗病性相关物质含量等生理指标。【结果】不同基因型与不同细胞质玉米都可以利用低浓度HMC毒素培养滤液诱导以增强其抗性;不同处理时期,植物抗病性相关酶活性呈现不同的动态变化;不同的诱导处理均导致抗病性反应的产生,对C细胞质的预处理效果好于N细胞质,且具有浓度效应。【结论】低浓度HMC毒素培养滤液预处理后,刺激了POD、PAL酶活性提高和MDA含量下降,以此来启动玉米本身的防卫系统,因钝化作用而抑制侵染,当再接种高浓度HMC毒素培养滤液后,抗病性相关酶得到了进一步激活,使C细胞质玉米对HMC敏感性降低,从而玉米叶片呈现出抗病性。
[Objective] The influence of filtrated culture fluid of HMC toxin on inducing disease-resistance and its related enzymes in specific host maize （Zea mays L.） leaves was studied. [Method] The various pathogenicities were detected by the detached leaf method on the specific host maize leaves of two pairs of homokaryon （B37 and C103） inbred lines to search effective dilution factor for inducing, and changes of disease resistance-related enzymes were measured after inoculation. [Result] The disease resistance was enhanced in different genotypes and cytoplasms induced by low concentration filtrated culture fluid of HMC toxin, the effect on C cytoplasm was higher than that on the N cytoplasm. Activities of disease resistance-related enzymes showed dynamic changes in different periods, and influences of disease-resistance were led by the different concentrations. [ Conclusion] POD and PAL activity were increased and MDA content was decreased after pretreatment with filtrated culture fluid （1 ： 50 or 1 ： 60 ） of HMC toxin in order to start up maize defense system.for itself. It played as a passivation to restrain the HMC infection. After inoculating by high concentration filtrated culture fluid of HMC toxin, the disease resistance-related enzymes were activated further. So, the sensitivity of C cytoplasm to HMC was reduced and disease resistance was represented.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第12期4307-4313,共7页
Scientia Agricultura Sinica
基金
河北省自然科学基金资助项目(C2006000744)
国际科技合作资助项目(2006DFB02480)
河北省科学技术研究与发展计划项目(07297162D)
农业部948资助项目(2008-Z20)
关键词
玉米小斑病菌C小种
毒素培养滤液
苯丙氨酸解氨酶
过氧化物酶
丙二醛
Helminthosporium maydis race C （HMC）
Filtrated culture fluid of toxin
phenylalanine ammonialyase （PAL）, Peroxidase （POD）
Malondialdenvde （MDA）
