摘要
目的：通过研究大鼠半乳糖性白内障晶状体中氯通道蛋白-3（chloride channel protein．3，CLC-3）和水通道蛋白-1（aquaporin-1，AQP-1）表达水平的变化，探讨CLC-3和AQP-1与白内障发病的关系。方法：将半乳糖注射Wistar大鼠单侧眼球后，裂隙灯下观察晶状体混浊情况，分别在造模后不同时间点摘取模型组和对照组大鼠的眼球，取出晶状体，运用实时荧光定量PCR方法检测各组晶状体中CLC-3 mRNA和AQP-1 mRNA含量的变化。结果：透明晶状体和半乳糖性自内障晶状体中均有CLC-3 mRNA和AQP-1 mRNA的表达；生理盐水对照组各时间点CLC-3 mRNA和AQP-1 mRNA表达无统计学差异；模型组于3、7、14d时CLC-3 mRNA表达逐渐增高，均高于同期对照组（P〈0．01），到14d时约达正常水平7倍，21、30d时又有所下降，均低于同期对照组（P〈0．01）；各时间点晶状体CLC-3 mRNA含量两两比较，差异均有极显著意义（P〈0．01）；模型组于3d时AQP-1 mRNA表达已下降，与同期对照组比较差异有显著性意义（P〈0．05），且随着白内障进一步发展，AQP-1 mRNA含量逐渐降低，均低于同期对照组（P〈0．01）；各时间点晶状体AQP-1 mRNA含量两两比较，差异均有极显著意义（P〈0．01）。结论：晶状体中CLC-3和AQP-1表达的变化可能与白内障的发生发展有着密切的联系。
Objective： To investigate the expression of chloride channel protein - 3 （ CLC-3 ） and aquaporin-1 （ AQP-1 ） gene in galactose - induced cataract and normal lens of rats, to determine whether CLC-3 and AQP-1 expression was related to the occus and progression of cataract. Methods： Galactose was injected retrobulbarly into Wistar rats. The opacity of lens was examined by slit lamp. The lens were extracted periodically from each experimental group and control group and real - time quantitative PCR techniques were used to determine the expression of CLC-3 mRNA and AQP-1 mRNA in lens during different cataract phases. Results： CLC-3 mRNA and AQP-1 mRNA were expressed in both lens of normal rats and cataract rats. During our experiment, the expression of CLC-3 mRNA and AQP-1 mRNA in lens of control group was not changeable obviously. CLC-3 mRNA in lens gragually increased at 3,7,14 days after injection of D - galactose and reached 7 - fold that of normal level at 14 days（P 〈 0.01 ）. CLC-3 mRNA level decreased gradually 14 days later and was less than the normal level after 21 days （P 〈 0.01 ） . AQP-1 mRNA in lens have began to increase at 3 days after injection of D - galactose（ P 〈 0.05 ） and AQP-1 mRNA level decreased persistantiy accompany to progression of cataract（ P 〈 0.01 ）. Conclusions： There is an overexpression of CLC-3 mRNA in early stages of galaetose induced cataract, and a downexpression of AQP-1 mRNA in the lens of eataraetous rats, which may play an importment role in the gataractgenesis.
出处
《实用医学进修杂志》
2008年第4期220-226,共7页
Journal of Practical Training of Medicine
