摘要
应用SSR技术对21个核桃品种和1个枫杨品种的基因组DNA进行遗传多样性的研究。用筛选出的10对引物对供试材料进行SSR分析,共扩增出67条清晰的谱带,不同引物扩增出的DNA条带数量在4～24条之间,分子量大小在85～300 bp之间,依据特征谱带,可直接鉴别出供试品种,其中引物WGA32的鉴别效率最高。对扩增出的67条带进行聚类分析,结果表明,普通核桃和枫杨亲缘关系较远,不同地域起源的品种界限不明显。
The genetic diversity of 21 walnut species （Juglans regia L. ） and Fengyang （Pterocarya stenoptera DC） were analyzed by SSR technique. 10 pairs of SSR primers＇were collected, and each primer pair produced 4 - 24 bands and 67 polymorphic bands were obtained. The results showed that the number of DNA fragments by different primers changed from 4 to 24, while molecular sizes changed from 85 to 300 bp. The tested cultivars could be directly identified according to the special bands, and the efficiency of WGA32 was the best. The results of cluster and analysis of 67 alleles showed that the normal walnut and Fengyang were not significantly related to each other, with no obvious boundary line between the walnut species from different regions.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2008年第4期51-55,共5页
Journal of Hebei Agricultural University
基金
河北省林业局资助项目
