摘要
本试验于１９９６年对来自美国、加拿大和欧洲的１０６个小麦矮腥黑穗病菌（ＴＣＫ）和小麦网腥黑穗病菌（ＴＣＴ）菌瘿进行了冬孢子网脊高度值测量和自发荧光显微学观察。结果表明，网脊高度指数值（ＬＩ－Ｒ）可以准确地鉴别不同来源的ＴＣＫ菌瘿，准确率达９８．６％，而来源于美国和加拿大的ＴＣＴ菌瘿则有不同程度的错判。自发荧光显微学方法可准确地鉴别不同来源的ＴＣＴ菌瘿，准确率达１００％，同时也能较准确地鉴别不同来源的ＴＣＫ菌瘿，但不适合鉴别保存时间过长或已死亡的ＴＣＫ菌瘿。结合这两个特征。
106 sori of Tilletia controversa Kuhn (TCK) and Tilletia caries Tul. (TCT), intercepted from import wheat shipment of different countries during last 21 years, were identified with the methods established in ChinaUS Joint Research, i.e., teliospore reticulation depth and autofluorescence microscope. Results showed that reticulation depth of teliospores could be used for the identification of sori of TCK and TCT from Europe as well as USA in many cases, and with some error identifications for TCT. Autofluorescence microscope could also be used for the identification of TCK and TCT from different origins, especially for TCT (100% accurate), but perhaps was not suitable for the delimitation of dead TCK. The combination of these two methods should be more reliable in the identification of these two important wheat disease pathogens if sori were found.
出处
《植物检疫》
北大核心
1998年第1期1-7,共7页
Plant Quarantine
关键词
小麦
矮腥黑穗病菌
网黑穗病菌
菌瘿鉴定
Tilletia controversa, Tilletia caries, Reticulation depth, Autofluorescence, Identification of sori
