摘要
目的观察不同浓度阿奇霉素对哮喘小鼠气道炎症细胞和辅助性T细胞2(Th2)型细胞因子IL-4、IL-5的影响。方法BALB/c小鼠40只随机分为5组:A组(哮喘模型组,8只)、B组(小剂量阿奇霉素治疗组,8只)、C组(中剂量阿奇霉素治疗组,8只)、D组(大剂量阿奇霉素治疗组,8只)、E组(正常对照组,8只)。A、B、C、D组小鼠于第1、13天分别给予卵清蛋白(OVA)20μg和氢氧化铝1mg的混悬液腹腔注射致敏,于第21～30天每天给予雾化吸入2%OVA生理盐水溶液建立哮喘模型,第14～30天,A组每天激发前30min给予生理盐水0.2ml腹腔注射作为阳性对照组,B、C、D组每天激发前30min分别给予阿奇霉素50、100、150mg.kg-1.d-1腹腔注射,均为每天1次。E组以生理盐水代替OVA致敏和激发。所有动物于第31天处死,收集支气管肺泡灌洗液(BALF),制备肺组织石蜡切片,用计数板检测BALF中炎症细胞总数及嗜酸性粒细胞(EOS)数,用酶联免疫吸附法(ELISA)检测BALF中IL-4、IL-5水平。结果A、B、C、D组的BALF中炎症细胞总数、EOS数及IL-4、IL-5水平较E组明显增高,HE染色显示A、B、C、D组支气管黏膜周围有EOS、淋巴细胞等炎症细胞浸润,EOS分别与IL-4、IL-5呈显著正相关。阿奇霉素可明显降低哮喘小鼠的气道炎症和IL-4、IL-5水平。结论阿奇霉素具有明显抗哮喘气道炎症的作用,可通过抑制Th2反应,减轻慢性气道炎症。
Objective To observe the effects of azithremyein （AZM） with different concentration on airway inflammation and Th2 cell cytokines IL-4, IL-5 in asthmatic mice models. Methods Forty BALB/e mice were randomly divided into five groups ： group A （ asthmatic model group, n = 8 ）, group B （ low dose AZM treated group, n = 8 ）, group C （ moderate dose AZM treated group, n = 8 ）, group D （ high dose AZM treated group, n =8）and group E （eontrel group, n =8）. Mice in groups A,B,C and D were respectively sensitized on day 1 and 13 by intraperitoneal injection of Ovalbumin （OVA） 20 μg together with aluminum hydroxide [ Al （OH） 3 ] 1 mg and challenged from day 21 to day 30 by inhalation of 2% OVA to establish a murine model of asthma. From day 14 to day 30, the animals in group A were treated by intraperitoneal injection of 0. 9% NaCl at 30 rain before sensitized each day, the animals in groups B, C, D were respectively treated intraperitoneal injection of lower dose AZM（50 mg/kg per day） , moderate dose AZM（ 100 mg/kg per day） , higher dose AZM（ 150 mg/kg per day） at 30 rain before sensitized each day. Group E was sensitized and challenged by 0. 9% NaCl. All the animals were killed on day 31. The broncho-alvedar lavage flu- id （BALF） was collected and the paraffin sections of pulmonary tissues were made. The levels of IL-4, IL-5 in BALF was determined by ELISA and the total WBC and EOS were counted. Results Compared with the control group（ group E） ,the total WBC and the numbers of eosinophils as well as IL-4, IL-5 in BALF of asthmatic model groups （group A, B, C, D） were significantly increased. Inflammatory cells infiltration including lymphocytes and eosinophils were observed around airway and vessels in group A, B, C, D. There was a significant correlation between EOS and IL-4, IL-5. After the treatment of AZM , the airway inflammation and the levels of IL-4, IL-5 were decreased compared with the group A, B, C, D. Condusions AZM can suppress the inflammation of airway in routine asthmatic model. The mechanism may be explained by that AZM could inhibit the response of Th2, so that it could decrease the airway inflammation.
出处
《中国厂矿医学》
CAS
2008年第6期646-648,共3页
Chinese Medicine of Factory and Mine
