摘要
目的:用HPLC法测定巴洛沙星中有关物质和降解产物。方法:色谱柱为Prodigy 5μODS3100A(150×4.60mm 5 micron),乙腈-0.05mol.L-1枸橼酸溶液(25∶75)为流动相,流速为1.0mL.min-1,检测波长为294.0nm。结果:巴洛沙星与各杂质均能有效的分离。结论:该方法结果准确,操作简单,灵敏度高,专属性强,适用于巴洛沙星的有关物质测定。
Objective： To establish an HPLC method for The determination of related substances and degradation products in balofloxacin. Method：HPLC with Prodigy 5u ODS3 100A （150 × 4.60mm 5 micron）as column, acetonitrile and 0. 05mol · L^-1 citric acid （25：75 ） as mobile phase, flow rate ： 1.0mL · min^ - 1, UV detector at 294. Onm. Result： Balofloxacin and all of the related substances can be separated efficiently. Conclusion： The method is accurate, simple, specific and exclusive,it could be used for determination of related substances in balofloxaein.
出处
《化学工程师》
CAS
2008年第12期46-47,53,共3页
Chemical Engineer
