实时荧光定量PCR检测孕妇血浆中胎儿RhCcEe基因
被引量:2
摘要
Rh血型系统在临床输血中的重要性仅次于ABO血型系统,Rh血型不合可引起溶血性输血反应及新生儿溶血病(HDN)。Lo等研究证实孕妇血浆或血清中含有胎儿DNA,国外已用游离胎儿DNA开展了非创伤性产前诊断Rh基因的研究。我们曾用PCR扩增孕妇血浆中胎儿DNA,以检测胎儿RhCcEe基因。此外,因实时荧光定量(FQ)-PCR比PCR技术的灵敏度高,我们尝试用FQ—PCR检测孕妇血浆游离胎儿DNA的RhCcEe基因型。
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2008年第12期1365-1366,共2页
Chinese Journal of Laboratory Medicine
基金
浙江省医药卫生科学研究基金资助项目(2007A043)
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共引文献7
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同被引文献17
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引证文献2
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二级引证文献4
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