摘要
克隆了减蛋综合征病毒（ＥＤＳＶ）中国分离株基因组ＨｉｎｄⅢ－Ｅ片段，并分析了其核苷酸序列。该片段位于基因组中央５８．７～６８．９物理图谱单位（ｍｕ），片段全长共３１９４ｂｐ。其中含有１个完整的和２个不完整的开放性读码框架（ＯＲＦ），分别编码ｐＶⅢ蛋白、１００Ｋ蛋白基因Ｃ端和纤维蛋白Ｎ末端部分。ｐＶⅢ蛋白基因由７５３个碱基（ｎｔ）组成，编码２５０个氨基酸（ａａ），编码产物的分子量为２８５００。氨基酸水平的同源性比较表明，ＥＤＳＶ的ｐＶⅢ蛋白与禽腺病毒１型（ＦＡＶ１）及人和其他动物腺病毒ｐＶⅢ蛋白的同源性为２８％～３６％，而与羊腺病毒（ＯＡＶ）的同源性高达５２％，提示ＥＤＳＶ与ＯＡＶ间的亲缘关系更近。按照人腺病毒基因组结构的特征，在ｐＶⅢ和纤维蛋白基因之间，应为Ｅ３区，但该序列只有２４５个ｎｔ组成，序列中保留了ＴＡＴＡＢｏｘ和ｐｏｌｙＡ信号。但除了２个仅编码４９ａａ和３２ａａ多肽的ＯＲＦｓ外，不编码任何产物，且核苷酸序列与其他腺病毒的Ｅ３区无同源性，证实此区无明显Ｅ３区样结构。
The complete nucleotide sequence of a Hind ⅢE DNA fragment of an egg drop syndrome virus isolate, EDSV2 isolated from China, was determined. It was shown that the fragment contained the hexon associated protein pVⅢ gene, E3 region and part of fiber gene coding the Nterminal tail region. Homology studies revealed that the gene coding structural protein PVⅢ existed on the left and the gene coding fiber protein on the right of the sequenced fragment. According to the generally highly conversed nature of the genetic organization of adenovirus genomes, the sequence between them would have included the equivalent of the human adenoviral E3 region. However, no ORFs of the E3 region of human adenoviruses were identified. The intergenic region between the pVⅢ and the fiber genes accounted for only 245 nt and these appeared to be incapable of coding any protein. Thus, there was no E3 region in the expected location of EDSV. At the map of 65.1 mu an open reading frame of 753 nt encoding the protein pVⅢ was identified which consisted of 250 aa with an expected molecular weight of 285 000. The amino acid sequence showed a homology of 28% to 36% to the FAV1 and human and other animal adenoviruses. However, its amino acid homology with ovine adenovirus was determined to be 52%.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第1期27-31,共5页
Chinese Journal of Veterinary Science
基金
病毒基因工程国家重点实验室基金
