摘要
目的:探讨电针"水沟"穴对梗死脑动脉血管细胞内信息转导通路的影响。方法:Wistar大鼠56只,随机分为模型组24只、针刺组24只和空白对照组8只,前两组又分为大脑中动脉阻滞后1、3、6h3个时相组,每组8只。采用Longa腔内线栓法阻滞大鼠右侧大脑中动脉,复制脑梗死模型。电针"水沟"穴(15Hz,0.1mA),持续20min。镜下剥离脑表面动脉血管组织,应用蛋白质竞争结合分析法测定胞内三磷酸肌醇(IP3)含量;采用薄层定量分析法测定胞内二酰基甘油(DAG)含量。结果:①模型1、3、6h3个时相组胞内IP3及DAG含量均明显高于空白对照组(P<0.01),针刺1、3、6h3个时相组胞内IP3及DAG含量均明显低于相应模型组(P<0.01)。结论:电针"水沟"穴调节脑梗死大鼠脑血管舒缩运动从而促进侧支循环的效应可能与细胞内IP3及DAG等信使物质有关。
Objective To observe the effect of electroacupuncture （EA） on inositol triphosphate （IP3） and diacylglycerol （DAG） contents in cerebral arteries of the cerebral cortex in rats with cerebral ischemia （Cl） ,so as to explore its effect on intracellular signal transduction pathway. Methods Fifty-six Wistar rats were randomly divided into control （n = 8）, Cl （n = 24） and EA （n = 24） groups, and the later 2 groups were further separately divided into 1 h, 3 h and 6 h subgroups （Cl-1 h, 01-3 h, Cl-6 h, EA-Cl-1 h, EA-Cl-3 h and EACl-6 h）, with 8 cases in each. CI model was made by occlusion of the middle cerebral artery. EA （15 Hz, 0. 1 mA） was applied to ＂Shuigou＂ （GV 26） for 20 rain. The cerebral arteries in the cerebral shallow layer were collected for assaying intracellular DAG content by thin-layer chromatography （TLC） and IP3 content by competitive protein binding assay （OPBA）. Results Compared with control group,the levels of IP3 and DAG in cerebral vascular smooth muscle were significantly increased in model 1 h , 3 h and 6 h subgroups （P〈0.01）. Compared with the corresponding model subgroups,the levels of IP3 and DAG were significantly decreased in EA-Cl-1 h, EA-Cl-3 h and EA-Cl-6 h subgroups （P〈0.01）. Conclusion EA of GV 26 can effectively inhibit Cl-induced increase of intracellular IP3 and DAG levels in rat cerebral arteries, which may contribute to its effect in modulating vascular motor to ameliorate cerebral microcirculation.
出处
《针刺研究》
CAS
CSCD
2008年第6期392-396,共5页
Acupuncture Research
基金
国家自然科学基金资助项目(No.30472243)
关键词
脑梗死
电针
三磷酸肌醇
二酰基甘油
Cerebral ischemia
Electroacupuncture Cerebral inositol triphosphate
Diacylglycerol
