摘要
目的:利用响应面法对超声提取苜蓿皂苷的工艺条件进行了优化。方法:研究了超声波条件下影响提取的几个因素,包括超声时间、超声温度、超声功率、固液比等,并通过响应面法优化工艺条件。结论:根据中心组合设计原理采用四因子三水平的响应面分析法,通过对各因子显著性和交互作用的分析,得出了超声提取苜蓿皂苷的最佳工艺条件为:超声时间22min,超声温度43℃,超声功率403W,固液比1∶51(g/ml),此时苜蓿皂苷的得率为4.53%。
Obiective：To optimize the extraction technique for total saponins from alfalfa by supersonic wave,the single factor tests and response surface method were used. Method： The factors including supersonic wave time, supersonic temperature, supersonic power, and the ratio of bittermelon to solvent , were studied. The best extraction method was found through the response surface method. Result： The reponse surface method with 4 factors and levels was adopted. The results showed that the optimun conditions were that supersonic wave time of 22 min, extracting temperature of 43℃ ,supersonic power of 403W, and the ratio of bittermelon to solvent of 1：51（g/ml）. Under such conditions the extraction rate of total saponins could be up to 4.53 % at one - time extraction.
出处
《生物技术》
CAS
CSCD
2008年第6期65-68,共4页
Biotechnology
关键词
响应面法
提取
苜蓿
总皂苷
response surface method
extraction
bittermelon
total saponins
