摘要
目的观察局灶性脑缺血后海马和缺血边缘区星形胶质细胞过度增殖对微循环的影响。方法将大鼠随机分为假手术组、缺血组和干预组,缺血组和干预组采用线栓法制备大脑中动脉栓塞模型,缺血组侧脑室采用ALZET微渗透泵给予0.2%DMSO,干预组给予细胞周期抑制剂roscovitine,假手术组不插入线栓,不给与任何药物干预。缺血7d后于股静脉注入FITC标记的葡聚糖标记血管,小鼠抗大鼠单克隆抗胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)抗体标记星形胶质细胞;激光共聚焦三维成像显示胶质细胞于微循环之间的关系。结果缺血同侧海马和缺血边缘区在GFAP阳性细胞增多的同时,局部微血管血流灌注明显减少,应用细胞周期抑制剂roscovitine抑制星形胶质细胞增生可以明显增加局部微血管的血流灌注。结论脑缺血后缺血边缘区和海马的反应性星形胶质细胞增生与微循环灌注减少密切相关。
Objective To observe the effect of excessive astrocytic proliteration on tocal microcircu lation in hippocampus and ischemia boundary zone after middle cerebral artery occlusion （MCAO） Methods Rats were randomly divided into the sham-operated group, ischemia group and roscovitine group. Cerebral ischemia was induced by occluding the middle cerebral artery for lh in the ischemia and roscovitine-treated groups. 0. 2% DMSO was infused into the lateral ventricle by ALZET micro-osmotic pump in the ischemia group. The roscovitine-treated group was given cell cycle inhibitor roscovitine. NO drug intervention was given in the sham-operated group Vascular marker FITC-dextran was injected into femoral vein 7d after ischemia. Immunofluorescent stain was used to show astrocytes. Three-dimensional laser scanning confocal imaging was performed to showthe relationship between astrocytes and microcircula：tion. Result GFAP-positive cells were increased in the ipsilateral hippocampus and ischemic boundary zone, with local microvascular perfusion decreased significantly~ Inhibition of glial cell proliferation by roscovitine infusion significantly increased local microvascular perfusion. Conclusion Prolifrative astrocytes are closely related to microcirculation in hippocampus and boundary zone after ischemia.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2008年第6期571-575,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家杰出青年基金(30725019)
国家科技部973重大专项(2006CB5040502)
国家自然科学基金(30600187)
