摘要
在具有高效护水功能石蜡油的保护下进行了人宫颈癌细胞(HeLa细胞)的低能离子注入及真空处理,用荧光定量PCR的方法分别研究了真空和30keVN+的不同注入剂量下,细胞中p53基因和c-fos基因的mRNA表达变化。结果表明,上述两个基因的表达与真空及低能离子注入之间存在着剂量关系。
The relationship between gene expression and low-energy ion implantation has been studied.Because vacuum is prerequisite in low-energy ion implantation, mineral oil was used to protect cells from water evaporating. In this study, HeLa cells were implanted by low-energy ions （30 keV N^＋） at different doses, and p53 gene and c-fos gene were studied with real-time quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×10^14ions/cm^2.
出处
《原子核物理评论》
CAS
CSCD
北大核心
2008年第4期385-390,共6页
Nuclear Physics Review
基金
国家十五科技攻关资助项目(2001BA302B-03)~~
