摘要
目的筛选口腔鳞状细胞癌和癌前病变细胞中与氧化应激相关的差异表达基因,探讨氧化应激损伤与口腔癌发生的相关性及其作用机理。方法采用核酸微阵列技术,对口腔鳞状细胞癌细胞株Tca8113、KB及口腔癌前病变细胞株DOK细胞的基因表达谱进行比较研究,检测、筛选口腔鳞状细胞癌中与氧化应激相关的差异表达基因。结果与氧化应激相关的差异表达基因有28条。与氧化应激关系较密切的差异表达基因有9条,如过氧化物酶、超氧化物歧化酶、谷胱甘肽合成酶、谷胱甘肽S-转移酶、硫氧还原酶及硒蛋白等。另外,细胞角蛋白17、细胞角蛋白10及细胞角蛋白7在Tca8113和KB细胞中表达明显增高。结论氧化应激损伤可能通过某种信号通路引起细胞角蛋白发生变化,从而导致口腔鳞状细胞癌的发生。氧化应激损伤可能与口腔鳞状细胞癌的发生密切相关。
Objective To identify differentially expressed genes related to oxidative stress in oral squamous cell carcinoma and oral precancerous lesion cells and investigate the mechanism and relationship between oxidative damage and oral cancer development. Methods cDNA microarray analysis was used to examine the differences in gene expression between oral carcinoma cell lines Tca8113, KB and oral precancerous lesion cell line DOK. Results Twenty-eight differentially expressed genes related to oxidative stress were detected in oral squamous cell carcinoma and oral precancerous lesion cells. Among them,9 genes were separately up-regulated from two folds to six folds, including PRDX, SOD, GSS, GST, TXNRD, SEPX1, etc. High expression of cytokeratin 10, 17 and 7 were also observed in the cells of oral squamous cell carcinomas. Conclusion Oxidative damage may be related to the carcinogenesis of oral cancer, and oxidative stress damage may activate some signal pathway related to cytokeratinization which leads to carcinogenesis.
出处
《北京口腔医学》
CAS
2008年第6期309-311,共3页
Beijing Journal of Stomatology
