摘要
利用大鼠胚胎中脑神经细胞进行原代培养（微团试验）研究氟化钠（ＮａＦ）的细胞毒性及其对神经元分化的影响。细胞毒性试验提示ＮａＦ的半数存活抑制浓度（ＩＣＶ５０）为１５．０μｇ／ｍｌ。染毒组的细胞集落形成率降低，细胞体积小，细胞间神经纤维减少等形态学变化，其中前者呈剂量—效应关系，表明ＮａＦ可抑制胚胎神经细胞的分化，半数分化抑制深度（ＩＣＤ５０）为３．０μｇ／ｍｌ，半数存活抑制深度与半数分化抑制浓度的比值（Ｖ／Ｄ）大于５．０。表明抑制细胞分化可能是ＮａＦ致发育危害的重要作用机理。
The micromass culture of rat embryo midbrain cells was applied to study in vitro develomental toxicity of sodium fluorode,cytotoxicity and inhibition of microcolone formation as endpoints.The results showed that high levels of sodium fluoride was cytotocical to embryo midbrain cell in culture and inhibited the clone formation with dose-effect relationship.The concentration of inhibituon for 50% cell differenciation(ICd50) was about 3.0μg/ml according to the dose-effect relationship curve. The data suggest that inhibition of cell differentiation may be one of action mechamisms of high levels sodium fluoride′s adverse effects on developing embryo brain.
关键词
氟化钠
微团培养
细胞分化
发育毒性
Fluoridw Micromass Cultrue Cell Differentiation Developmemtal Toxicity
