摘要
本文建立了反相高效液相色谱法测定Levofloxacin（左旋氧氟沙星）的血药浓度方法，样品经液－液提取后，C(18)柱上分析，流动相为甲醇：0．01M磷酸盐缓冲液：0．5M四丁基溴化铵，紫外检测波长294nm，线性范围0．5～4．0μg／ml，平均回收率为97．17％，日内误差＜7％，日间误差＜8％，本法具有简便、灵敏、快速、准确的优点，已用于药代动力学的研究。
A RP-HPLC method for determination of levofloxacin in serum was developed.The sample was extracted by one step liquid-liquid extraction followed by separation on C18 column with a mobile phase of mixture of methanol:0.01M phosphate buffer:0.5M tetrabutyl ammonium bromide.The UV detector wavelength was set at 294nm.The standard curve was linear in 0.5~4.0μg/ml range.The average recovery was 97.17 % and the variations of inter-day and intra-day were less than 7% and 8%, respectively.The method is simple,sensitive,accurate and has been applied to pharmacokinetic studies in human.
出处
《中国药房》
CAS
CSCD
1998年第1期28-29,共2页
China Pharmacy
