摘要
目的 探讨一种可评价具有携氧功能的抗休克液体的实验动物模型。方法SD大鼠在麻醉状态下做35%、45%、55%和65%放血失血性休克模型，以乳酸林格液（LR）、全血进行复苏，观察其对失血性休克大鼠平均动脉血压（mean arterial blood pressure,MAP）、左心室收缩压（ldft intraventricular systolic pressure,LVSP）、左心室压力上升或下降的最大速率（the maximal change rate of left intraventricular pressure,±dp/dtmax）的影响，同时观察血气指标变化 和存活率的变化。结果 35%失血性休克后，输注LR和全血均可使MAP恢复到90-100mmHg水平，同时LR和全血对恢复休克后动物血流动力学指标、血气指标以及对存活率的影响无显著性差异；45%和55%失血性休克后，单纯LR输注不能使MAP恢复到90-100mmHg水平，且对休克动物的血流动力学指标和血气指标以及存活率的影响明显不及输注全血以后效果好，在55%失血性休克时差异更大。65%放血后动物很快死亡，休克程度太重，不能作为评价模型。结论 55%失血性休克大鼠可作为评价具有携氧液体抗休克作用的实验动物模型。
Objective To establish an experimental animal model which can be used to evaluate the antishock effects of an oxygen-carrying shock resuscitation fluid.Methods SD rats were used to make hemorrhagic shock model by 35%,45%,55%and 65% hemorrhage respectively under anesthesia.Effects of Lactated Ringer＇s solution（LR）and whole blood on mean arterial blood pressure（MAP）,left intraventricular systolic pressure（LVSP）,the maximal change rate of left intraventricular pressure（±dp/dtmax）in those 4 types of hemorrhagic shock rats were observed.Blood gas and 24-hour survival rate were also observed.Results For rats with 35% hemorrhagic shock,LR and blood infusion recovered MAP to 90-100mmHg,but had no significantly difference in hemodnamic parameters and blood gas.In 45% and 55% hemorrhagic shock,blood infusion improved hemodynamic parameters and blood gas singificantly better than LR infusion.The 24-hour survival rat in blood treatment group were higher than LR group.Animal in 65% hemorrhagic group died soon after hemorrhage,and could not be used as the evaluation animal.Conclusion Rats with 55% hemorrhagic shock is an ideal experimental model to evaluate the antishock effect of oxygen-carrying shock resuscitation fluid.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第1期64-66,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30600228)
教育部长江学者和创新团队发展计划(IRT0712)
重庆市自然科学基金(CSTC2007BB5078)~~
关键词
失血性休克
乳酸林格液
全血
大鼠
hemorrhageic shock
Lactated Ringer＇s solution
blood
rats
