摘要
目的:研究乳铁蛋白对神经病理性疼痛大鼠的镇痛作用。方法:SD大鼠40只,随机分为5组(n=8)。结扎L5、L6脊神经(SNL)模型制成后第5天,分别腹腔注射生理盐水1ml(组Ⅰ)或乳铁蛋白0.5g/kg(组Ⅱ)、1g/kg(组Ⅲ)、2g/kg(组Ⅳ)溶于生理盐水1ml腹腔注射,腹腔内注射乳铁蛋白1g/kg+纳洛酮2mg/kg(组Ⅴ)。观察给药前和给药后不同时间点各组大鼠机械性痛敏实验的压力阈值(PPT)和热敏实验的潜伏期(PWL)。结果:与组Ⅰ比较,组ⅡPPT和PWL差异无统计学意义;组Ⅲ、Ⅳ给药后30～180min PPT升高,30～60min PWL延长。组Ⅴ与组Ⅰ比较,PPT和PWL差异无统计学意义,与组Ⅴ相比,组Ⅲ、组Ⅳ给药后30～180min PPT增加,30～60min PWL延长。结论:乳铁蛋白通过阿片受体介导,对神经病理性疼痛大鼠产生镇痛作用。
Objective： To investigate the analgesic effect of lactoferrin （LF） on neuropathic pain in rats with lumbar 5 and lumbar 6 spinal nerve ligation （SNL）. Methods ： The SNL model was established by modified spinal nerve ligation 5 days before drugs administrated. Forty rats were randomly divided into five groups（n =8）, ie Saline lml（group Ⅰ ）, LF 0.5 g/kg （group Ⅱ ）, 1 g/kg（groupⅢ）, 2 g/kg （ group Ⅳ ）, or LF 1 g/kg ＋ Naloxone 2 mg/kg （ group Ⅴ ） was given i. p.. The paw pressure threshold （PPT） and the paw withdrawal latency（PWL） were measured in both hind paws before and after drugs administration. Results： PWL and PPT were significantly increased in group Ⅲ and group Ⅳ as compared with that in group Ⅰ . There was no significant difference in PWL and PPT among group Ⅰ , Ⅱ and Ⅴ. Comparing with groupⅤ, PFF and PWL were significantly increased in groupmand group Ⅳ. Conclusion： LF exerts analgesic effects via the opioid receptor activation in rats with neuropathic pain.
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2008年第6期360-363,共4页
Chinese Journal of Pain Medicine
