摘要
以喜树果为原料,对匀浆法提取喜树碱和10-羟基喜树碱的工艺进行了研究,确定了最佳的工艺条件:提取溶剂为体积分数55%的乙醇,匀浆时间为8 m in,料液比为1∶15(g/mL)。在此工艺条件下,喜树碱和10-羟基喜树碱的提取率分别为0.801‰和0.546‰。将该法与超声提取、回流提取、常温冷浸提取、水浴振荡提取进行了比较,结果表明,匀浆提取具有得率高、省时间等方面的优势,是一种高效提取喜树碱和10-羟基喜树碱的方法。
The method of homogenate extraction of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata seed was studied, and comparisons of this method with other extractive methods such as sonieation, reflux, soakage, shaking in water bath were condueted. For the homogenate extraction, the optimum technology parameters were found as following：homogenate for 8 min with 55% ethanol at a ratio of material to liquid of 1： I5（g： mL）. By using the optimized parameters, the extraction yield of camptotheein and 10-hydroxycamptothecin was 0. 801‰ and 0. 546‰, respectively. Moreover, homogenated extraetion required less extraction time and had high yield, which was an efficient method for extracting eamptothecin and 10-hydroxycamptothecin.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第6期1088-1090,1124,共4页
Natural Product Research and Development
基金
国家科技支撑计划项目(2006BAD18B04)
关键词
匀浆
喜树果
喜树碱
10-羟基喜树碱
homogenate
Camptotheca acuminata seed
camptotheein
10-hydroxycamptothecin
