摘要
目的:考察乌头须根中总生物碱的最佳提取工艺条件。方法:酸碱滴定法测定乌头须根中总生物碱含量,以总生物碱提取率为指标,采用L9(3)^4正交实验法筛选乌头须根总生物碱的最佳提取工艺。结果:乌头须根总生物碱含量为1.094%,影响提取的主次因素为:乙醇浓度〉提取次数〉提取时间〉乙醇用量;优选得到的最佳提取工艺为A3B1C3D3,即以8倍量80%的乙醇提取3次,每次1.5小时。结论:乌头须根总生物碱含量较高,提取工艺条件稳定、经济、可行。
Objective:To determine the extract technology of total alkaloids from the fibrous root of Aconitum carmichaeli Debx. Methods:The method of acid-alkali titrations was applied to determine the contents of total alkaloids,the optimum conditions were investigated by orthogonal design and the extraction ratio of total alkaloids was used as the index.Results:The contents of total alkaloids from the fibrous root of Aconitum was 1.094%,factors influencing the extracting efficiency were as follow:the concentration of alcohol〉the frequency of extract〉the time of extracting〉the quantity of Alcohol;the optimum extracting condition was A_3B_1C_3D_3,that was adding eight times amount of 80% alcohol and refluxing and extracting for 3 h (totally extracting for 3 times).Conclusions:The contents of total alkaloids from the fibrous root of Aconitum carmichaeli is high,and the extracting condition has good reproducibility, and it is workable,economic and convenient.
出处
《现代生物医学进展》
CAS
2008年第11期2109-2111,共3页
Progress in Modern Biomedicine
关键词
乌头须根
总生物碱
含量测定
提取工艺
The fibrous root ofAconitum carmichaeli Debx
Total alkaloids
Contents determines
Extract technology