摘要
目的:观察藤茶总黄酮(Tengcha flavonoids,TCF)对大鼠肝纤维化的防治作用。方法:66只雄性SD大鼠被随机分为5组:正常对照组(10只),正常喂养;模型组(14只):采用四氯化碳(CCL4)皮下注射诱导大鼠肝纤维化模型;TCF治疗组分为低、中、高剂量组(每组14只):在造模的同时,每日分别予以TCF25mg/100g、50mg/100g、100mg/100g体重灌胃。于第12周末检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)的水平以及肝组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量变化。结果:与模型组比较,TCF各剂量组血清中ALT、AST、HA、LN、PCⅢ、CⅣ的水平显著下降(P<0.05);SOD活性明显增高,MDA的含量明显降低(P<0.05)。结论:TCF对CCL4实验性肝纤维化大鼠有较好的防治作用,其作用机制可能与抗氧化、抗脂质过氧化有关。
Objective： To investigate the preventive effect of Tengcha flavonoids＇s （TCF） on hepatic fibrosis of rats. Method： 66 male SD rats were randomly divided into 5 groups which include healthy control group （10 rats）, the hepatic fibrosis model group （14 rats） and three treatment groups of TCF （low doses group, medium doses group, high doses group, 14 rats in each group）. For healthy control group, the rats were fed by normal food. For model group, the experimental models of hepatic fibrosis were induced by hypodermical injection of carbon tetrachloride （CCL4）. For the treatment groups： the rats were administrated with the same way of model group, at the same time, taking TCF as follow： low doses group （25mg/100g body weight）, medium doses group （50mg/100g body weight）, high doses group （100mg/100g body weight）. Twelve weeks later, the level of serum Alanine aminotransferase （ALT）, Aspartate aminotransferase （AST）, Hyaluronic Acid （HA）, Laminin （LN）, Procolagen Type Ⅲ （PCⅢ） and Collagen Type Ⅳ （CⅣ） were assayed, and hepatic tissue activity of superoxide dismutase （SOD）, and hepatic tissue contents of malondialdehyde （MDA） were measured in all groups. Results： Compared with model group, the level of serum ALT, AST, HA, LN, PCⅢ and CⅣ in TCF group were significantly decreased （P〈0.05）, MDA also decreased obviously, while the SOD activity of TCF group rose clearly. Conclusion： TCF has good preventive and therapeutic effects on the hepatic fibrosis induced by CCLA, and its mechanism is possibly associated with anti-oxidation and antilipid-peroxidation.
出处
《现代生物医学进展》
CAS
2008年第12期2445-2447,共3页
Progress in Modern Biomedicine
基金
国家自然基金资助项目(30771135)
湖南省自然科学基金资助项目(06jj4106)
关键词
大鼠
藤茶总黄酮
肝纤维化
超氧化物歧化酶
丙二醛
Rat
Tengcha flavonoids
Hepatic fibrosis
Superoxide dismutase
Malondialdehyde
