摘要
利用紫外、硫酸二乙酯、氯化锂和亚硝基胍复合诱变的方法,选育到一株高产黄色素的红曲霉突变株MYM2。经过稳定性实验证明,诱变得到的菌株稳定性较好,液态发酵试验黄色素色价达到100U/mL以上,黄色素色调达到3.5左右。此黄色素在300nm^600nm波长之间只有一个在410nm附近的最大吸收峰,在pH3~8之间稳定性较好。当pH小于3时,红曲黄色素不稳定,黄色素溶液变混浊,放置后有沉淀产生。
The Monascus mutant with high yield of yellow pigment was obtained by using conventional relevant mutation techniques, e.g., treating with physical mutagens（such as UV light） and chemical substances （such as N-methyl-N′-nitro-N-nitrosoguanidine）. The yeUow pigment was scanned from 300 nm to 600 nm with UV spectrometer, the maximal absorption was determined at 410 nm. The growth characteristic of Monascus mutant is stable, the yellow pigment value and colour hue in liquid fermentation can reach 100 U/mL and 3.5 respectively. The yellow pigment is stable from pH 3 to pH 8, but the precipitation appeared as the pH of the pigment solution lower than 3.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第12期1909-1914,共6页
Microbiology China
关键词
红曲霉
突变株
黄色素
色价
色调
Monascus, Mutant, Yellow pigment, Pigment value, Colour hue
