摘要
采用Agilent Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm),以甲醇-水(60¨40)为流动相,流速1.0ml/min,检测波长254nm,建立高效液相法(HPLC)测定血浆及尿液中阿普唑仑浓度。结果表明,HPLC法测定阿普唑仑和内标物的出峰时间是5.1min和8.73min,分离效果较好;阿普唑仑质控3种浓度(4.0μg/ml,8.0μg/ml,16.0μg/ml)日内和日间的RSD,血浆样品均小于6%,尿样均小于7%,血浆回收率为91.15%～93.69%,尿回收率为86.79%～90.11%,血浆及尿样品线性范围为2.0～64.0μg/ml,相关系数为0.9907。血浆和尿样品中阿普唑仑的最低检出限为2.0μg/ml。此条件可以对中毒药物阿普唑仑进行快速检测并定量,方法简便,准确。
To use the HPLC determination method to detect the concentration of Alprazola from human plasma and urine by Agilent Eclipse XDB-C18 （4.6mm× 150mm, 5μm） and math and water （60：40）were used as the mobile phase. Flow 1.0 ml/min,and the detection wavelenghth was 254nm. In the HPLC conditions, the retention times for internal standard Alprazola and diazepam were 5. lmin and 8. 73min respectively. With three quality control concentrations of （4.0μg/ml, 8.0μg/ml, 16.0μg/ml）, Plasma samples are less than 6 % and urine samples are less than 7%. The accuracy and plasma or urine of the recovery rates were 91. 15%-93.69% for plasma and 86.79 %-90.11 % for urine. The standard calibration curves of concentrations and peak area were liner in the concentration ranges of 2. 0 - 64. 0μg/ml, and the correlation efficiencies were 0. 9907. The condition can quickly detect and quantity the alprazola. The method is very simple and accurate.
出处
《广西科学》
CAS
2008年第4期411-413,共3页
Guangxi Sciences
