摘要
在采用超滤膜-树脂吸附乙醇一次洗脱联用技术提取茶树花多酚工艺得到粗提物的基础上,探索了不同体积分数乙醇水溶液梯度洗脱法制备高纯度茶树花多酚的方法,分离纯化得到了各洗脱物,应用HPLC法分析了各洗脱物的儿茶素的组成,并采用清除.OH及DPPH.方法比较了各洗脱物的抗氧化性能。结果表明,收集10%、20%、30%和40%乙醇洗脱液浓缩干燥后可得到得率为84.19%,质量分数为92.25%的茶多酚精制品,比一次用体积分数为70%乙醇水溶液洗脱得到多酚质量分数84.32%的样品纯度高。其中体积分数为20%乙醇的洗脱组分多酚质量分数为97.71%;各洗脱组分中不同儿茶素得到有效富集分离,它们对.OH及DPPH.清除率均呈量效关系,都高于未分离纯化的样品,其中体积分数为20%乙醇的洗脱组分中,EGCG质量分数最高为67.39%,咖啡碱得到有效去除,其对自由基清除率最高,可作为高级抗氧化剂使用。
In order to utilize waste tea flowers （ Camellia sinensis L O Kuntze） and develop an industrialized way to refine tea flower polyphenols, a novel method for obtaining high purity of tea flower polyphenols by various ethanol concentration gradient elution was established based on the previous technology of ＂uhra-fihration-resin absorption-ethanol elute extraction of tea flower polyphenols＂. The results showed that an average content as high as 92.25 % of refined tea flower polyphenols could be collected by gradient elution of 10%, 20%, 30% and 40% ethanol elute followed, whereas single elution of 70% ethanol elute only led to 84.32% content of tea flower polyphenols. Therefore, catechins in various eluant components were effectively separated. There was also a good correlation between their purity and scavenging efficiency of · OH and DPPH·, both of which were higher than that of the crude extracts. Ethanol （20%） elution led to the highest EGCG content of 67.39%, and effectively removed theine in the final products which may directly used as antioxidants.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2008年第12期107-111,共5页
Transactions of the Chinese Society for Agricultural Machinery
基金
江苏省农业科技攻关项目(项目编号:E2003344)
关键词
茶树花
茶多酚
儿茶素
梯度洗脱
抗氧化性
Tea flower, Tea polyphenols, Catechins, Gradient elution, Antioxidative activity
