摘要
目的对纳米银抗菌凝胶的体外细胞毒性进行评价,为建立医用纳米材料生物评价体系提供依据。方法采用MTT方法和琼脂覆盖法对纳米银抗菌凝胶的细胞毒性进行测试。结果纳米银凝胶的细胞毒性与银含量和接触时间以及测试方法有关。结论评价纳米医用材料的细胞毒性时需要考虑其用途、使用方式以及细胞毒性试验的方法等。
Object The evaluation for the cytotoxicity of nanoparticulate siliver antibacterial gel , its aim is to establish the evaluation method in vitro for the medical nanomaterial. Method The cytotoxieity of the nanoparticulate siliver antibacterial gel was examined by both MTT assay and Agar diffusion test. Result The cytotoxicity of the nanoparticulate siliver antibacterial gel was relative to the silver content, the contact time and test method. Conclusion while cytotoxicity of the medical nanomaterials was evaluated, all the facts such as the clinical purpose, fashion of the nanomaterials and the assay of cytotoxicity should be considered.
出处
《北京生物医学工程》
2008年第6期641-643,共3页
Beijing Biomedical Engineering
关键词
纳米银
MTT
细胞毒性
nanoparticulate siliver
MTT assay
eytotoxicity
