摘要
利用具有高分辨率和高灵敏度的毛细管电泳-电化学检测方法直接测定蔗糖及其水解产物的浓度随反应时间变化的规律．在HCI催化下，对不同温度时蔗糖的水解反应速率常数进行了测定，并求得反应活化能Ea为109．8kJ／mol．该方法比经典的旋光度测定方法更直观简便，结果令人满意．
The concentrations of sucrose and its hydrolysis products were directly monitored by capillary electrophoresis-electrochemical detection due to its high resolution and high sensitivity of detection. The rate constants of sucrose hydrolysis at different temperatures in 0.80 mol/L HCl and 0. 90 mol/L HCl were obtained from the concentration change of sucrose during the process of hydrolysis. At 25℃, 30℃ and 30 ℃, the measured rate constants of sucrose hydrolysis were 7. 81×10-3 min-1, 17. 44×10-3 min-1 and 33. 09×10-3 min-1 in 0. 80 mol/L HCl, and 11.18×10-3 min-1, 27. 32×10-3 min-1 and 44. 86×10-3 min-1 in 0. 90 mol/L HCl, respectively, from which the Ea value of 109. 85 kJ/mol was derived. Compared with the traditional polarimetric method, this method is more visualizable and convenient with satisfactory results.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1998年第1期31-34,共4页
Chemical Journal of Chinese Universities
基金
中国科学院长春应用化学研究所电分析化学开放实验室资助
关键词
毛细管电泳
电化学检测
蔗糖
水解反应
速率常数
Capillary electrophoresis, Electrochemical detection, Sucrose, Hydrolysis rate constant
