摘要
目的研究精浆中胱抑素C(CysC)的生物学特性及其检测意义,建立可供临床研究的参考数据。方法应用免疫胶体金技术对192例不育患者精浆及50例正常生育的男性精浆CysC进行对比检测,同时监测50例正常对照精浆标本在不同温度及时间段内CysC的变化情况。结果192例患者精浆标本中CysC最低值为6.53mg/L,最高值为43.56mg/L,平均值为(19.37±7.43)mg/L;50例正常对照精浆中最低CysC值为18.13mg/L,最高CysC值为28.84mg/L,平均值为(23.55±2.50)mg/L。少精子症为(15.92±4.96)mg/L、弱精子症为(15.62±2.13)mg/L、死精子症为(15.26±2.12)mg/L、无精子症为(13.88±2.38)mg/L、血精症为(15.52±5.92)mg/L、脓精症为(19.45±9.87)mg/L、精液不液化为(13.99±3.88)mg/L;患者各组间与正常对照组CysC值经统计学分析差异具有统计学意义(P<0.05)。精液中WBC数<5个/HP的CysC值最低为6.53mg/L,最高为34.82mg/L,平均值为(16.40±5.75)mg/L;精液中WBC数为5～10个/HP的CysC值最低为8.95mg/L,最高为39.54mg/L,平均值为(19.75±6.05)mg/L;精液中WBC数>10个/HP的CysC值最低为10.73mg/L,最高为43.56mg/L,平均值为(21.96±10.49)mg/L。结论少精子症、弱精子症、无精子症、死精子症、血精症、脓精症、精液不液化患者精浆CysC总体水平均明显低于正常对照组。炎症精浆中CysC值的变化提示CysC可能与其主要的细胞学来源及炎症部位相关。
Objective To investigate the clinical significance of CysC in seminal plasma and set up its reference range for clinical research. Methods The level of CysC in seminal plasma of 192 infertile men and 50 health men was detected by Immune colloidal gold technique Results For the level of CysC in seminal plasma 192 infertile men, the minimum concentration was 6.53mg/L , the maximum concentration was 43.56mg/L and the mean concentration was （19.37±7.43）mg/L. For the level of CysC in seminal plasma of 50 healthy men, the minimum concentration was 18.13mg/L, the maximum concentration was 28.84mg/L and the mean concentration was （23.55±2.50）mg/L. The mean level of CysC in seminal plasma of oligozoospermia, asthenospermia, thanatospermia, azoospermatism, hemospermia, pyospermia and dysliquated spermia were （15.92±4.96）mg/L, （15.62±2.13）mg/L, （15.26±2.12）rag/L, （13.88±2.38）mg/L, （15.52±5.92）mg/L, （19.45±9.87）mg/L, （13.99±3.88）mg/L respectively. There was a significant differetice between the patient group and healthy group （P〈0.05）. The values of the CysC in seminal plasma with white cell less than 5/HP were as follows：.the minimum 6.53mg/L, the maximum 34.82mg/L and the mean was （16.40±5.75）mg/L. For levels of the CysC in seminal plasma with below 5-10 white cell/HP, the minimum concentration 8.95mg/L, the maximum concentration 39.54mg/L and the mean concentration was （19.75±6.05）mg/L For levels of the CysC in seminal plasma with above 10 white cells/HP, the minimum 10.73mg/L, the maximum 43.56mg/L and the mean was （21.96±10.49）mg/L. Conclusion The level of CysC in oligozoospermia, asthenospermia, thanatospermia, azoospermatism, hemospermia, pyospermia and dysliquated spermia was all lower significantly than that in normal seminal plasma. The level of CysC in inflammatory seminal plasma might be associated with cytology source and inflammation place.
出处
《中国男科学杂志》
CAS
CSCD
2008年第11期13-16,共4页
Chinese Journal of Andrology
