摘要
目的:检测pin1 mRNA在非小细胞肺癌(NSCLC)患者循环血中的表达情况并探讨肺动静脉结扎顺序的影响及临床意义。方法:选择接受根治性手术的原发性NSCLC患者26例,按预定的结扎血管顺序术前将病例随机分为先结扎肺静脉组(PV组)和先结扎肺动脉组(PA组)。分别采集其术前(麻醉后)外周血标本、术中结扎肺静脉后近心端和远心端血标本及术后第7天外周血标本。选择10例需手术治疗的肺部良性疾病患者作为对照,以10例健康人作为阴性对照。运用实时荧光定量逆转录聚合酶链反应(RT-PCR)法定量检测患者循环血中pin1 mRNA的表达。结果:肺癌患者循环血中pin1 mRNA表达量显著高于肺部良性病变患者和正常人(分别为1.45～29.86,0.83～1.26,P<0.05);Ⅲ期患者pin1 mRNA的表达量高于Ⅰ～Ⅱ期(分别为18.48±1.64,10.57±1.05,P<0.05);有淋巴结转移者pin1 mRNA表达量高于无淋巴结转移者(分别为18.93±2.10,10.02±1.23,P<0.05);肺静脉远心端的表达量高于近心端的表达量(分别为30.56±1.37,20.31±1.48,P<0.05);手术后1周的表达情况显著低于术前(分别为20.68±1.17,29.43±2.62,P<0.05);PV组的pin1 mRNA在肺静脉近心端表达低于PA组(分别为9.95±0.91,14.71±1.64,P>0.05),在肺静脉远心端表达高于PA组(分别为16.84±2.36,13.36±1.78,P>0.05)。结论:pin1 mRNA在NSCLC患者血液中有高表达,肺癌根治术术中先结扎肺静脉可在一定程度上减少pin1 mRNA在血中的表达即减少癌细胞入血。
Objective To detect the expression of teracting 1 （pinl mRNA） in the circulation of non-small gate the effect of ligating pulmonary vein first or ligating haematogenous dissemination of cancer cells. Methods who underwent surgical resection with curative intention peptidylprolyl cis/trans isomerase NIMA-in. cell lung cancer （NSCLC） and to investi- pulmonary artery first during operation on Twenty-six consecutive were randomly assigned into pulmonary artery first-ligation group （PA group） and pulmonary vein first-ligation group （PV group）. Blood samples were collected just before and 7 days after the operation. During the lobectomy, blood samples of the proximal part and distal part of the pulmonary vein when it was ligated were collected. Another 10 patients with benign lung disease served as control subjects undergoing surgical resection, and 10healthy persos served as negative controls. All blood samples were subjected to real-time RT-PCR with pinl mRNA as the marker. Results Compared with the benign lung disease and healthy persons, pin1 mRNA in NSCLC was overexpressed（1.45-29.86 vs. 0.83-1.26 vs P〈0.05）pin1 mRNA in stage Ⅲ NSCLC and lymph node positive were significantly higher than in stage Ⅰ-Ⅱ and lymph node negative （18.48±1.64 vs.10.57±1.05,P〈0.05;18.93±2.10 vs.10.02±1.23,P〈0.05）Expression of pin1 mRNA in the distal part of the pulmonary vein was significantly higher than that of the proximal part （30.56±1.37 vs.20.31±1.48,P〈0.05）;the expression 7 days after the operation was significantly lower than that of preoperation （20.68±1.17 vs.29.43±2.62,P〈0.05）.There was no significant difference between the PA group and PV group （9.95±0.91 vs.14.71±1.64,P〉0.05;16.84±2.36 vs.13.36±1.78,P〉0.05）.Conclusion pinl mRNA was overexpressed in the circulation of NSCLC. Ligation of pulmonary vein before the ligation of the pulmonary artery may decrease the expression of pin1 mRNA in the circulation, which can prevent the release of tumor cells into the bloodstream.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第12期1132-1136,共5页
Journal of Central South University ：Medical Science
