摘要
目的建立复方首乌降脂胶囊中熊果酸的含量测定方法。方法采用HPLC法测定熊果酸的含量,色谱柱为C18柱,流动相为乙腈-pH4.0的0.02mol/L磷酸盐缓冲盐溶液(63:37),流速:1ml/min,检测波长:210nm。结果熊果酸的进样量在0.176μg～2.64μg范围内与峰面积成良好的线性关系(R=0.9992),加样回收率为97.52%,RSD为1.88%。结论该方法可用于复方首乌降脂胶囊的质量控制。
Objective To develop a method to determine ursolic acid in gold theragran capsules. Method HPLC conditions： A diamomsil C18 column, a mixture of acetonitrile-0. 02 mol/L pH 4. 0 phosphate buffer（63：37） as mobile phase, flow rate 1 ml/min, column temperature 40℃, and UV detection at 210 nm. Result The linear range of calibration curve was 0. 176 μg- 2.64 μg with the correlation coefficient of 0. 9992, the average recovery with RSD being 97. 52% ± 1.88%. Conclusion The method is sensitive, accurate and speedy, therefore suitable for product＇ s quality control.
出处
《中国现代药物应用》
2009年第2期1-2,共2页
Chinese Journal of Modern Drug Application
