摘要
研究了不同浓度卡那霉素(Km)对未转化黄瓜种子萌发及幼苗生长的影响,建立了转基因黄瓜卡那霉素抗性筛选体系。种子首先播在含Km 200 mg/L的1/2MS培养基中,能有效地抑制黄瓜幼苗生长,10 d后剪掉根系扦插移栽于蛭石中,能促使非转基因植株衰弱或死亡,有效地加速筛选。应用该筛选体系对花粉管通道法获得的抗虫转基因黄瓜T0种子进行初步筛选,获得Km抗性植株,抗性植株筛选率为1.6%,其中8.9%经PCR分子检测为阳性植株,初步证实EQKAM抗虫基因已整合到黄瓜基因组中。
Effects of Kanamycin of different concentration on cucumber seed germination and seedling growth were studied. Screening method for kanamycin resistant seedling was established. Seeds were sowed first in 1/2MS medium containing Km 200 mg/L, seedling growth could be effectively inhibited. After 10 d, root was cut and then transplanted the seedling into Km free vermiculite could enhance seedling declining and dying, and therefore enhanced screening of resistant plants. To seeds from pollen tube pathway transformation were screened by this method, 1.6 % of seedlings were tested as resistant, among them 8.9 % were tested as PCR negative. It primarily certified that EQKAM gene was combined into Cucumber gene group.
出处
《华北农学报》
CSCD
北大核心
2008年第6期54-57,共4页
Acta Agriculturae Boreali-Sinica
基金
天津市自然科学基金项目(05YFJMJC07000)
关键词
黄瓜
花粉管通道法
卡那霉素
Cucumber（ Cucumis sativu s L. ）
Pollen tube pathway
Kanamycin
