摘要
目的探讨维生素E(VitE)和N-乙酰半胱氨酸(NAC)两种抗氧化剂对甲醛(FA)所致小鼠学习记忆能力损伤的保护作用。方法将34只清洁级ICR小鼠随机分为对照组(生理氯化钠,n=8)、FA组(15 mg/kg,n=9)、FA(15mg/kg)+NAC(100 mg/kg)组(n=9)、FA(15 mg/kg)+VitE(100 mg/kg)组(n=8)。每日给药1次,连续7 d。第8天开始用六臂放射状水迷宫检测小鼠的学习记忆能力,连续检测7 d。并检测谷胱甘肽(GSH)、丙二醛(MDA)。结果FA组小鼠早期兴奋后期则静卧少动,其余各组小鼠无此现象。体重增长在各组小鼠间比较,差异无统计学意义(P>0.05)。经重复测量资料方差分析,发现FA组小鼠学习期和记忆期潜伏时间延长,差异有统计学意义(F=5.479和2.953,P<0.01);但学习期和记忆期的小鼠错误次数差异无统计学意义(P>0.05);FA组的GSH含量高于对照组,差异有统计学意义(P<0.05)。两个干预组的MDA低于FA组,但差异无统计学意义(P>0.05)。结论抗氧化剂对FA致小鼠学习记忆能力损伤有保护作用。
Objective To explore the effects of vitamin E and N-acetyl-cysteine （NAC） antioxidants on learning and memory ability impairment induced by formaldehyde in mice. Methods 34 ICR mice were divided into four groups： control group（NS,n =8）, FA group treated with FA （15 mg/kg,n =9）, （FA ＋NAC） group（n =9） treated with FA （15 mg/kg） and NAC（100mg/ kg）, and （FA ＋ VitE） group（ n = 8） treated with FA （ 15 mg/kg） and VitE （100 mg/kg）. All materials were intraperitoneaily in-jected once a day for 7 days. On the 8th day, the learning and memory ability of mice were tested by using water labyrinth task for 7 consecutive days. Glutathione hormone（GSH） and malondialdehyde（MDA） were detected. Results The mice in FA group were seen exciting at first, and then transformed gradually into inactive, which was not observed in other group. There were no differences of body weight gain and the ratios of brain to body weight among 4 groups. With Repeated Measures of General Linear Model, the latency of learning time and memory time in FA group were much longer than those in other groups （P〈0.01）. There were no differences in the index of wrong numbers in the learning time and memory time. GSH levels of brain tissue in FA group were much higher than those of the control group （P〈0.05）. MDA levels of brain tissue in FA group were much higher than those of the FA ＋ NAC group and the FA＋ VitE group, but there were no differences among them （P〉0.05）. Conclusion The learning and memory ability impairment of mice induced by formaldehyde could be prevented by antioxidants.
出处
《中国职业医学》
CAS
北大核心
2008年第6期471-474,共4页
China Occupational Medicine
基金
安徽省教育厅自然科学基金项目(2002kj145)
安徽省高等学校"十五"优秀人才计划资助项目
关键词
甲醛
抗氧化剂
学习记忆能力
Formaldehyde
Antioxidant
Learning and memory ability
