摘要
将灰毡毛忍冬茎段和叶片接种在MS培养基中,并在培养基中添加不同浓度的生长素2,4-D,6-BA,NAA,以此探讨茎段和叶片2种外植体在不同浓度的生长素组合中愈伤组织的诱导和增殖情况。结果表明:叶片诱导愈伤组织的效果比茎段好,并且叶片和茎段诱导愈伤组织的最佳培养基均为MS+2,4-D 1.0 mg/L+6-BA 0.5 mg/L,愈伤组织增殖培养的最佳培养基为MS+6-BA1.5 mg/L+NAA 0.5 mg/L。
The explants of different organs of Lonicera macranthoides Hand.-Mazz.were used to induce callus tissues in MS medium with different sorts and concentrations of growth regulating substances, including 2,4-D, 6-BA and NAA. The result indicated that the leaf was proper explants , the callus were easier to be induced in MS＋2,4-D 1.0 mg/L ＋ 6-BA 0.5 mg/L, and the callus propagation coefficient was the highest when the callus of Lonicera macranthoides were cultured in MS ＋6-BA 1.5 mg/L ＋ NAA0.5 mg/L.
出处
《西南农业学报》
CSCD
2008年第6期1690-1694,共5页
Southwest China Journal of Agricultural Sciences
基金
四川师范大学校级项目
关键词
灰毡毛忍冬
愈伤组织
诱导
增殖
Lonicera macranthoides Hand.-Mazz.
callus
induction
propagation
