摘要
背景:小肠黏膜下层作为一种组织材料有很好的支撑作用,其降解性、生物相容性良好,但存在降解速度快等弊端。将其与软骨细胞进行复合培养后,由于具有良好的生物相容性和细胞相容性,从而更利于提高再生组织的速度和质量。目的:以转化生长因子β1诱导骨髓间充质干细胞生成的软骨细胞作为种子细胞,小肠黏膜下层作为支架材料,观察细胞-小肠黏膜下层复合物在体内形成新生软骨组织的可能性。设计、时间及地点:完全随机对照动物实验,于2007-07/2008-07在南方医科大学珠江医院儿科中心实验室完成。材料:6只4月龄雄性新西兰大白兔,随机分成软骨细胞/小肠黏膜下层复合物实验组3只,单纯小肠黏膜下层对照组3只。方法:取生长状态良好的第3代的兔骨髓间质干细胞制成细胞悬液,培养基中加入软骨起源诱导因子(转化生长因子β110μg/L、地塞米松10-7mmol/L和维生素C50mg/L)培养3周。将细胞悬液种于小肠黏膜下层支架上形成软骨细胞-小肠黏膜下层复合物。实验组用诱导后的软骨细胞、小肠黏膜下层复合物移植到成年新西兰大白兔的耳郭软骨缺损部位,对照组以单纯小肠黏膜下层植入。主要观察指标:分别于种植后4,10,16周取缺损区行大体观察和组织学检查。结果:诱导软骨细胞-小肠黏膜下层复合物在植入体内16周时材料明显被吸收完全,且有软骨生成,与正常组织未见明显区别,但修复层软骨较薄。经苏木精-伊红染色,Massan染色,甲苯胺蓝染色检查证实为软骨组织。结论:经诱导的软骨细胞-小肠黏膜下层复合物在动物体内能够形成新生软骨组织,比单纯小肠黏膜下层修复效果好。
BACKGROUND： Small intestinal submucosa （SIS） plays a support role as a tissue engineering material and shows good degradability and biocompatibihty. However, the degradation speed is too rapid. The pace and quality of regenerated tissues are doomed to increase when SIS is combined with chondrocyte. OBJECTIVE： To investigate the possibility of using chondrocytes created from bone marrow mesenchymal stem cells （BMSCs） which induced by transforming growth factor-β1 as germ cells and SIS as bracket to form new cartilage and repair the rabbits＇ ear cartilage defects. DESIGN, TIME AND SETTING： A completely random controlled animal experiment was completed in Pediatrics Laboratory of Zhujiang Hospital, Southern Medical University between July 2007 and July 2008. MATERIALS： Six New Zealand male rabbits of four months old were divided into four two groups at random： chondrocytes/SIS compound group （n=3） and SIS control group （n=3）. METHODS： The third generation BMSCs that in good condition were prepared into cell suspension, and then culture medium was added with inducer factors which can form chondrocytes as germ cells： 10 μg/L transforming growth factor-131, 10 7 mmol/L dexamethasone and 50 mg/L ascorbic acid. After 3 weeks of the culture, the compound of after-induced chondrogenetic BMSCs and SIS was transplanted into the defects of ear cartilage in rabbits as the experimental group, while transplanted SIS without BMSC served as control group. MAIN OUTCOME MEASURES： At 4, 10, and 16 weeks after cultivation, general observation and histological examination were conducted on the samples of bone defect area. RESULTS： At 16 weeks after the compound of after-induced chondrogenetic BMSCs and SIS were transplanted, the materials had been completely absorbed and there were new cambium in the experimental group, which proved to be chondrocytes after hematoxylin-eosin stain, Massan stain and Toluidine blue dyeing. CONCLUTION： The compound of after-induced chondrogenetic BMSCs and SIS can engender new chondrocytes, so the repair effect is better than only transplanting SIS.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第45期8805-8809,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
