摘要
应用生物信息学手段,收集和整理了411个玉米产量及构成因子QTL相关信息,借助高密度玉米遗传图谱IBM2 2005 Neighbors,采用BioMercator2.1软件构建出含221个QTL的整合图谱。采用统合分析方法,在LOD值大于4.0时于玉米第6染色体上确定1个与穗数、粒重和单位籽粒产量相关的"一致性"QTL,由标记bnlg1732和bnl8.08c界定,间距14.51cM。从MaizeGDB网站下载"一致性"QTL区间内基因和标记的原始序列,通过NCBI网站在线软件BLASTx同源比对分析,初步获得3个产量位置候选基因。利用比较基因电子定位策略,将53个水稻和玉米产量相关基因定位到玉米IBM2 2005 Neighbors图谱上,在"一致性"QTL区间内发现1个控制水稻分蘖数基因,初步确定为产量位置候选基因。本文结果为确定有关玉米产量因子的QTL及其候选基因提供了信息。
A total of 411 quantitative trait loci （QTL） conferring grain yield and its components of maize was collected and analyzed using bioinformaties tool, and the integration map containing 221 QTL was constructed by BioMercator 2.1 software using Genetic Map IBM2 2005 Neighbors as reference. Furthermore, one consensus QTL conferring ear number, kernel weight and grain yield was identified on chromosome 6 using meta-analysis method with LOD score value ≥ 4.0, which was positioned between the markers bnlg1732 and bnl8.08c with map interval of 14.51 eM. The gene and marker sequences within the consensus QTL were downloaded respectively from website MaizeGDB, and 3 positional candidate genes were identified by comparing these sequences against other genes by software BLASTx in NCBI website. Using a synteny conservation approach based on comparative mapping between maize genetic map and rice physical map, a total of 53 rice and maize genes were projected onto maize genetic map IBM2 2005 Neighbors, and one gene involving in the expression of rice tilling was found in the consensus QTL, which was determined as the positional candidate gene for the grain yield of maize. The results provided useful information for identifying QTL conferring grain yield and its components and candidate genes in maize.
出处
《核农学报》
CAS
CSCD
北大核心
2008年第6期756-761,838,共7页
Journal of Nuclear Agricultural Sciences
基金
国际科技合作项目(2007DFA31010)
