摘要
以"岗山3号"蔺草成熟种子作为外植体,研究了不同激素浓度及组合对愈伤组织诱导及植株再生的影响。结果表明,在2,4-D浓度为2.0mg/L,6-BA浓度为0.5mg/L时,蔺草种子的愈伤组织诱导率最高,为74.7%;在IAA浓度为0.5mg/L,KT浓度为2.0mg/L时,蔺草种子的愈伤组织分化率最高,为83.3%;IAA与KT的比值对再生芽和再生根形态影响较明显。
The mature seeds of Juncus effusus L. eultivar Gangshan No. 3 were different concentrations and combinations of hormones on the callus induction and used as exp!ants to investigate the effects of plant regeneration. The results indicated that the callus induction rate of mat rush＇s seed was the highest on the medium with MS ＋ 2.0mg/L 2,4-D ＋ 0.5mg/L BA, and the induction rate reaches 74.7% ; the callus differentiation rate was the highest on the medium with MS ＋ 2.0mg/L KT ＋ 0.5mg/L IAA, and the differentiation rate was 83.3%. In addition, regeneration of root and bud formation was affected significantly by the medium with different ratio of IAA and KT.
出处
《核农学报》
CAS
CSCD
北大核心
2008年第6期803-805,共3页
Journal of Nuclear Agricultural Sciences
基金
浙江省科技厅支撑和引导计划(2007C22009)
浙江万里学院校级重点项目
