摘要
目的:比较18FDG和99Tcm-MIBI双核素心肌显像评价经心内膜心肌内移植骨髓间充质干细胞的效果。方法:成年小型猪8头,麻醉后利用心血管支架置入技术栓塞冠状动脉左前降支远端建立心肌梗死模型,随机均分为2组。造模2周后将分离纯化的猪自体骨髓间质干细胞通过NOGA电机械标测系统经心内膜注射移植至细胞组梗死区,对照组仅注射胎牛血清。移植前及移植后4周分别行18FDG和99Tcm-MIBI双核素心肌显像。结果:移植前两组均可见左室心尖部及前壁呈显著放射性缺损。与对照组比较,移植后4周细胞组梗死区18FDG和99Tcm-MIBI双核素心肌显像所呈现的缺血/梗死节段数均明显减少(P<0.05)。与移植前比较,移植后4周对照组无明显变化(P>0.05);细胞组梗死区18FDG和99Tcm-MIBI双核素心肌显像所呈现的缺血/梗死节段数均明显减少(P<0.05),且99Tcm-MIBI显像示减少幅度更大(P<0.05)。结论:经心内膜心肌内注射移植骨髓间充质干细胞可显著改善心肌灌注损伤,增加存活心肌,且99Tcm-MIBI显像改善效果优于18FDG显像。
OBJECTIVE： To assess outcome of bone marrow mesenchymal stem cell transplantation in the myocardium via the endocardium using dual-isotope （^18FDG and ^99Tc^m-MIBI） imaging （DISA）. METHODS： Following eight adult mini-swines were anesthetized left anterior descending coronary artery were embolized by intravascular stent implantation to establish myocardial infarction models. The swines were equally and randomly assigned into two groups. Two weeks after model induction, autologous bone marrow mesenchymal stem cells following purification were injected into the infracted region via the endocardium using the NOGA electric machine measurement system. Swines in the control group were only treated with fetal bovine serum. Before and 4 weeks after transplantation, each swine were examined by DISA. RESULTS： Left ventricular apex and anterior wall showed significant radioactive defects in both groups prior to transplantation. Compared with the control group, ischemia/infarcted segments displayed by DISA were significantly reduced in the cell group at 4 weeks following transplantation （P 〈 0.05）. Compared with pre-transplantation, no significant difference was detected in the control group at 4 week following transplantation （P 〉 0.05）. In the cell group, ischemia/infarcted segments displayed by DISA were significantly reduced （P 〈 0.05）, and the decrease was greater by using the ^99Tc^m-MIBI imaging （P 〈 0.05）. CONCLUSION： Bone marrow mesenchymal stem cell transplantation in the myocardium via the endocardium can significantly improve the myocardial perfusion damage and increase surviving myocardium. The improvement is better using DISA compared with ^18FDG imaging.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第51期10028-10032,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
陕西省社发公关基金[2004K14-G6(1)]~~
